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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Article DOI: 10.3791/54863-v • 09:58 min • November 29th, 2016
November 29th, 2016 •
1, 1, 1
1Department of Biological Sciences, University of Illinois at Chicago

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We describe here an optimized protocol of fluorescent Electrophoretic Mobility Shift Assays (fEMSA) using purified SOX-2 proteins together with infrared fluorescent dye-labeled DNA probes as a case study to tackle an important biological question.

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Electrophoretic Mobility Shift Assay Infrared Fluorescent Dye-labeled Oligonucleotides Protein-DNA Interactions Non-radioactive Probes Molecular Biology Biochemistry DNA Target Sequence Native Polyacrylamide Gel Tris Borate EDTA TBE Glycerol Mini Protein Gel System Ammonium Persulfate TEMED Acrylamide Bis Polymerization Oligonucleotide Design Infrared Fluorescent Dye Modification Annealing
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