Órgão Cultura e Whole Mount Imunofluorescência Coloração de rato Wolff Dutos
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January 13, 2017
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Nós apresentamos um método para isolamento e cultura do duto de Wolff rato (WD). Também demonstramos um procedimento detalhado para toda a imunocoloração monte da cultura / WDs recentemente isolados com anticorpos marcados fluorescentes. Em conjunto, estas técnicas permitem o estudo do desenvolvimento WD, de enrolamento, e a diferenciação.
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Cite this Article
Kumar, M., Tanwar, P. Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts. J. Vis. Exp. (119), e55134, doi:10.3791/55134 (2017).
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