Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Shuo Li; Sumana Raychaudhuri; Shigeki Watanabe

doi:10.3791/55664

Journal

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Biologia

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Flash-e-Freeze: uma nova técnica para capturar Membrane Dynamics com Electron Microscopy

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Flash-e-Freeze: uma nova técnica para capturar Membrane Dynamics com Electron Microscopy

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13,871 Views

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10:01 min

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May 01, 2017

DOI:

10.3791/55664-v

DOI:

10.3791/55664-v

•

10:01 min

May 01, 2017

•

16 Views

Shuo Li*^1,2, Sumana Raychaudhuri*¹, Shigeki Watanabe^1,3

¹Department of Cell Biology,Johns Hopkins School of Medicine, ²Department of Biochemistry and Molecular Biology,Johns Hopkins Bloomberg School of Public Health, ³Solomon H. Snyder Department of Neuroscience,Johns Hopkins School of Medicine

Transcrição

Summary

Nós desenvolvemos uma nova técnica de microscopia electrónica, de "flash-e-congelamento", que permite a visualização da dinâmica da membrana com ms resolução temporal. Esta técnica combina a estimulação optogenetic de neurónios com congelamento de alta pressão. Aqui, demonstramos os procedimentos e descrever os protocolos em detalhe.