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Isolation og respiratoriske målinger af mitokondrier fra Arabidopsis thaliana
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Isolation og respiratoriske målinger af mitokondrier fra Arabidopsis thaliana

Article DOI: 10.3791/56627-v • 09:54 min • January 5th, 2018
January 5th, 2018 •
1, 1, 1, 2, 3, 1, 1
1ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, 2School of Biological Sciences, Flinders University, 3ARC Centre of Excellence in Plant Energy Biology, University of Western Australia

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Mitokondrier er kun en lille procentdel af anlægget celle, skal de være renset for en række undersøgelser. Mitokondrier kan isoleres fra en bred vifte af anlæg organer ved homogenisering, efterfulgt af differentiale og tæthed gradient centrifugering at opnå et højt oprenset mitokondrie brøkdel.

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Biokemi sag 131 Arabidopsis thaliana mitokondrie isolation tæthed gradienter renhed fraktionering respiratorisk målinger blå native-polyacrylamid gelelektroforese (BN-side) Sodium dodecyl sulfat-polyacrylamid gelelektroforese (SDS-PAGE) western blotting
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