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Journal / Behavior / A Burrowing/Tunneling Assay for Detection of…
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Behavior

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JoVE Journal Behavior
A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae
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A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae

Article DOI: 10.3791/57131-v • 09:04 min • March 27th, 2018
March 27th, 2018 •
1,2, 1, 1
1Department of Biosciences, Rice University, 2Yale Medical School

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The protocol describes a simple assay to identify Drosophila melanogaster larvae that are experiencing hypoxia under normal atmospheric oxygen levels. This protocol allows hypoxic larvae to be distinguished from other mutants that show overlapping phenotypes such as sluggishness or slow growth.

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Burrowing/tunneling Assay Detection Hypoxia Drosophila Melanogaster Larvae Tissue Hypoxia Mutations Slow Growth Poor Growth Sluggishness Oxygen Deficiency Physiological Processes Damaged Airways Trachea Burrow Into Food Tunnel Into Substratum Control Genetic Crosses Experimental Genetic Crosses Egg-lay Dishes Timed Egg Collections Darkness Grape Agar Plates Yeast Paste Incubate 25 Degrees Celsius
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