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JoVE Journal Bioengineering
Mikroinjektion af Western majs-rodorm Diabrotica virgifera virgifera, embryoner for kønscelleoverførsel Transformation, eller CRISPR/Cas9 genom-redigering
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Mikroinjektion af Western majs-rodorm Diabrotica virgifera virgifera, embryoner for kønscelleoverførsel Transformation, eller CRISPR/Cas9 genom-redigering

Article DOI: 10.3791/57497-v • 07:42 min • April 27th, 2018
April 27th, 2018 •
1, 1, 1, 1, 1
1Department of Entomology and Plant Pathology, North Carolina State University

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Her præsenterer vi protokoller for indsamling og microinjecting precellular vestlige majs rodorm embryoner med henblik på udførelse af funktionelle-genomisk assays kønscelleoverførsel transformation og CRISPR/Cas9-genom-redigering.

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Bioteknologi spørgsmål 134 embryonale mikroinjektion funktionel genomforskning kønscelleoverførsel transformation transgenese CRISPR/Cas9 genom-redigering vestlige majs-rodorm
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