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Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

Article DOI: 10.3791/57673-v • 07:37 min • June 7th, 2018
June 7th, 2018 •
1, 1,2, 3, 1,2, 3,5, 1,2,4
1Research Laboratory of Electronics, Massachusetts Institute of Technology, 2Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 3Department of Medicine, University of Pittsburgh, 4Department of Biological Engineering, Massachusetts Institute of Technology, 5Vascular Medicine Institute, University of Pittsburgh

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In this research, we demonstrate a label-free neutrophil separation method from clinical airway secretions using closed-loop operation of spiral inertial microfluidics. The proposed method would expand the clinical in vitro assays for various respiratory diseases.

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Label-free Neutrophil Enrichment Patient-derived Airway Secretion Closed-loop Inertial Microfluidics Pulmonary Diseases Research Pneumonia Asthma Cystic Fibrosis Chronic Obstructive Pulmonary Disease Technique Isolate Enrich Patient Neutrophil Chemical Disturbance Kyungyong PhD Student Laboratory PDMS Precursor Base Curing Agent Micro Machine Aluminum Mold Petri Dish Degas Vacuum Desiccator Oven Knife Fluid Access Holes Biopsy Punch Device Tape Forceps Air Plasma
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