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En Flow flowcytometri-baserede analyse til måling af membran potentiale i hjerte Myocytes efter hypoxi/Reoxygenation
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En Flow flowcytometri-baserede analyse til måling af membran potentiale i hjerte Myocytes efter hypoxi/Reoxygenation

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Vi præsenterer her, en protokol, der bruger JC-1 farvestof til at vurdere mitokondrielle membran celler efter at være udsat for iltsvind/reoxygenation med eller uden en beskyttende agent.

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Adfærd sag 137 mitokondrie permeabilitet overgangen pore mitokondrielle membran potentiale JC-1 assay reperfusion skade hjerte myocytes flowcytometri
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