Mensuração ecocardiográfica dos parâmetros diastólicos do ventrículo direito no mouse

Overview

This article describes two positions for obtaining the apical four-chamber view in mice, which allows for the quantification of right ventricular function. The techniques provide comparable results and can be used interchangeably, improving visualization rates in mice.

Key Study Components

Area of Science

• Neuroscience
• Cardiovascular Imaging
• Animal Models

Background

• Visualization of the apical four-chamber view is typically achievable in only 65-70% of mice.
• Combining both tilting positions enhances the success rate of this visualization technique.
• Light sedation and proper positioning are crucial for effective imaging.
• Ultrasound coupling gel is necessary for optimal transducer performance.

Purpose of Study

• To improve the visualization of the apical four-chamber view in mice.
• To compare the effectiveness of two different tilting positions.
• To enable consistent quantification of right ventricular function.

Methods Used

• Light sedation of mice on a heated platform.
• Application of electrode gel for heart rate and temperature monitoring.
• Use of depilatory cream to remove chest hair.
• Application of ultrasound coupling gel to the transducer tip.

Main Results

• Both tilting positions significantly increased the success rate of obtaining the apical view.
• Results were comparable between the two positions used.
• Technique can be applied interchangeably without loss of accuracy.
• Enhanced imaging allows for better assessment of right ventricular function.

Conclusions

• The combined use of tilting positions is effective for imaging in mice.
• This technique can be reliably used to assess cardiac function.
• Improved visualization methods can lead to better research outcomes in cardiovascular studies.

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