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Journal / Biochemistry / Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA…
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Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA 分析
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Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA 分析

Article DOI: 10.3791/58079-v • 07:54 min • August 29th, 2018
August 29th, 2018 •
1,2,3, 1, 1, 1, 1,2, 1
1The Smidt Heart Institute, Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences

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在这里, 我们提出了一个协议, 执行 polysome 分析的孤立灌注小鼠心脏。我们描述的方法, 心脏灌注, polysome 分析, 并分析 polysome 分数的基因, miRNAs, polysome 蛋白质组。

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生物化学 问题 138 缺血再灌注 动态蛋白质组 polysome 剖面 miRNA mRNA 新合成的蛋白质 蛋白质翻译 蔗糖密度梯度沉淀 孤立小鼠心脏灌注 Langendorff 灌注,蛋白质 组 学。
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