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Immunology and Infection

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JoVE Journal Immunology and Infection
Co-immunoprecipitation analyse til at studere funktionel interaktioner mellem receptorer og enzymer
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Co-immunoprecipitation analyse til at studere funktionel interaktioner mellem receptorer og enzymer

Article DOI: 10.3791/58433-v • 09:40 min • September 28th, 2018
September 28th, 2018 •
1, 2, 2
1Pulmonary Department, The Chaim Sheba Medical Center, 2Columbia Center for Translational Immunology, Department of Medicine, Columbia University Medical Center

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Vi præsenterer her, en protokol for co-immunoprecipitation og en på perle enzymatisk aktivitet assay samtidig undersøge specifikke protein domæner af plasmamembran receptorer for både enzym rekruttering og enzymaktivitet bidrag.

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Immunologi og infektion sag 139 Co-immunoprecipitation enzymatisk aktivitet protein-protein interaktion protein domæner fosfatase programmeret død-1 PD-1
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