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Isoleren, Sequencing en analyseren van extracellulaire MicroRNAs van menselijke mesenchymale stamcellen
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Isoleren, Sequencing en analyseren van extracellulaire MicroRNAs van menselijke mesenchymale stamcellen

Article DOI: 10.3791/58655-v • 10:55 min • March 8th, 2019
March 8th, 2019 •
*1, *1, 1,2, 1, 1, 1,2
1The Interdisciplinary Nanoscience Centre, Aarhus University, 2Department of Molecular Biology and Genetics, Aarhus University
* These authors contributed equally

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Dit protocol laat zien hoe om te zuiveren van extracellulaire microRNAs van cel voedingsbodems voor kleine RNA bibliotheek constructie en de volgende generatie sequencing. Verschillende kwaliteitscontrole controlepunten worden beschreven om lezers te begrijpen wat u kunt verwachten wanneer u werkt met lage input monsters als exRNAs.

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Genetica kwestie 145 mesenchymale stamcellen cellen extracellulaire RNAs extracellulaire blaasjes ribonucleproteins exosomes microRNAs klein RNAs volgende generatie sequencing kleine RNA bibliotheek bouw
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