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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Article DOI: 10.3791/58918-v • 06:14 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1
1Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center

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This article demonstrates the technique of expanding a traditional, two-dimension (2D) electrospun nanofiber mat into a three-dimension (3D) scaffold through the depressurization of subcritical CO2 fluid. These augmented scaffolds are 3D, closely mimic cellular nanotopographic cues, and preserve the functions of biologic molecules encapsulated within the nanofibers.

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Two-dimensional Electrospun Nanofiber Mats Three-dimensional Scaffolds Depressurization Subcritical CO2 Fluid Aqueous Solutions Chemical Reactions Multiple Step Processes Encapsulated Biological Molecules Hydrophobic Polymers PCL Dichloromethane DMF Electrospinning Apparatus Syringe Blunt Needle Rotating Steel Drum Ground Collector Direct-current High-voltage Power Supply Syringe Pump
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