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人类肠道器官脂滴形成特征化和定量的荧光测定

Article DOI: 10.3791/60150-v • 10:12 min • October 13th, 2019
October 13th, 2019 •
1,2,3, 1,2, 1,2
1Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University, 2Regenerative Medicine Center, University Medical Center Utrecht, Utrecht University, 3Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University

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该协议描述了在脂肪酸刺激下人体肠道器官中脂液滴（LD）形成的表征测定。我们讨论了这种测定如何用于LD形成定量，以及如何用于影响LD形成的药物的高通量筛选。

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医学， 问题 152， 人肠道器官， 原发细胞培养， 活检， 脂液滴， DGAT1， 共聚焦显微镜
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