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Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
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Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Article DOI: 10.3791/60225-v • 07:38 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1,2
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, 2Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

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Astrocytes are morphologically complex cells, exemplified by their multiple processes and bushy territories. To analyze their elaborate morphology, we present a reliable protocol to perform intracellular Lucifer yellow iontophoresis in lightly fixed tissue.

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Visualizing Astrocyte Morphology Lucifer Yellow Iontophoresis Evaluation Astrocyte Neuronal Interactions Synapse Disease Steady States Mouse Model Cell Population Brain Region Sharp Electrode Preparation Resistance Borosilicate Glass Single Barrel Electrode Micropipette Puller Storage Of Electrodes Lucifer Yellow Dye Solution Electrode Filling Process Capillary Action Electrode Holder Manipulator Silver Wire Dye Ejection Test Brain Slice Fixation Glass Bottom Dish PBS Solution
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