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Immunology and Infection

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Live Cell Imaging en Cryo-Electron Tomography toepassen op Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Article DOI: 10.3791/60693-v • 09:12 min • March 10th, 2020
March 10th, 2020 •
1, 1,2, 3, 1,2, 1
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston

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Beeldvorming van bacteriële cellen is een opkomende systeembiologiebenadering gericht op het definiëren van statische en dynamische processen die de functie van grote macromoleculaire machines dicteren. Hier wordt integratie van kwantitatieve live celbeeldvorming en cryo-elektronentomografie gebruikt om legionella pneumophila type IV secretiesysteemarchitectuur en -functies te bestuderen.

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Immunologie en infectie kwestie 157 Legionella pneumophila legionairsziekte luchtweginfectie intracellulaire bacteriële pathogenen type IV secretiesysteem Dot/Icm secretiesysteem levende celbeeldvorming spatiotemporale dynamiek kwantitatieve fluorescentiemicroscopie cryo-elektronentomografie cellulaire structuren alleallic uitwisseling
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