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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Article DOI: 10.3791/60961-v • 08:05 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences

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The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.

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Drosophila Larval Testes Pupal Testes Cell Division Live Tissue Analysis Fluorescence Microscopy Native Physiological Environments Live Imaging Dissection Mounting Visual Demonstration Testes Identification Testes Isolation Tissue Imaging Animals Preparation Tools And Media Filter Syringe Glass Slide Larvae And Pupae Agitation
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