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JoVE Journal Immunology and Infection
Construcción de mutantes en el serotipo 1 Streptococcus pneumoniae cepa 519/43
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Construcción de mutantes en el serotipo 1 Streptococcus pneumoniae cepa 519/43

Article DOI: 10.3791/61594-v • 06:06 min • September 11th, 2020
September 11th, 2020 •
1, 2, 3, 2, 1
1Faculty of Infections and Tropical Diseases, Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Division of Medicine, University College London, 3Division of Infection and Immunity, University College London UCL

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Aquí, describimos una cepa 519/43 del serotipo 1 de S. pneumoniae que puede modificarse genéticamente utilizando su capacidad para adquirir ADN de forma natural y un plásmido suicida. Como prueba de principio, se hizo un mutante isogénico en el gen de la neumolisina (ply).

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Inmunología e infección Número 163 Serotipo 1 S. pneumoniae Mutagénesis competencia natural Cinturón de meningitis
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