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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Article DOI: 10.3791/61603-v • 09:09 min • March 5th, 2021
March 5th, 2021 •
1, 1, 1
1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University

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Here, we show the use of traditional dark-field microscopy to monitor the dynamics of gold nanorods (AuNRs) on cell membrane. The location and orientation of single AuNRs are detected using ImageJ and MATLAB, and the diffusive states of AuNRs are characterized by single particle tracking analysis.

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Darkfield Microscopy Gold Nanorods Cell Membrane Diffusion Dynamics Single Particle Tracking Analysis Translational Dynamics Rotational Dynamics Complex Biological Systems Ethanol Dipped Glass Coverslip Cell Culture Dish Cell Culture Medium Phenol Red Cell Suspension CTAB Coated Gold Nanorods Humidified Atmosphere Glass Slide Sealing
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