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Journal / Biochemistry / MS2亲和力纯化与革兰阳性细菌中的RNA测序相结合…
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Biochemistry

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JoVE Journal Biochemistry
MS2亲和力纯化与革兰阳性细菌中的RNA测序相结合
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MS2亲和力纯化与革兰阳性细菌中的RNA测序相结合

Article DOI: 10.3791/61731-v • 08:34 min • February 23rd, 2021
February 23rd, 2021 •
1, 2, 2, 1, 1, 1
1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke

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MAPS 技术已开发出来，以仔细审查体内特定监管 RNA 的目标。感兴趣的 sRNA 标有 MS2 贴录器，通过 RNA 测序实现其 RNA 合作伙伴的共同纯化及其标识。此修改后的协议特别适合革兰氏阳性细菌。

Tags

收回， 第 168 期， MAPS， 监管 RNA， RNA 测序， Targetome， 监管网络， 共同净化， MS2 阿普塔默， 革兰阳性细菌， 金黄色葡萄球菌
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