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Isolering og funktionel vurdering af stamceller fra brystkræft fra celle- og vævsprøver

Article DOI: 10.3791/61775-v • 07:03 min • October 2nd, 2020
October 2nd, 2020 •
1,2, 1,2, 1, 1,2, 1,2,3,4
1London Regional Cancer Program, 2Department of Anatomy & Cell Biology, Western University, 3Department of Oncology, Western University, 4Lawson Health Research Institute

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Denne eksperimentelle protokol beskriver isoleringen af BCSC'er fra brystkræftcelle- og vævsprøver samt in vitro - og in vivo-assays , der kan bruges til at vurdere BCSC-fænotype og funktion.

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Kræftforskning udgave 164 brystkræftstamcelle (BCSC) fluorescensaktiveret cellesortering (FACS) kolonidannende assay mammosfæreanalyse 3D-kulturmodel in vivo tumormodel
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