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Journal / Immunology and Infection / Kontaktfri co-kulturmodel til undersøgelse af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kontaktfri co-kulturmodel til undersøgelse af medfødt immuncelleaktivering under luftvejsvirusinfektion
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
Journal (Immunology and Infection)
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Imaging the Human Immunological Synapse
Journal (Immunology and Infection)
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Kontaktfri co-kulturmodel til undersøgelse af medfødt immuncelleaktivering under luftvejsvirusinfektion

Article DOI: 10.3791/62115-v • 07:36 min • February 28th, 2021
February 28th, 2021 •
*1, *1, 1, 2, 3, 1,4, 1,4, 5, 2,6, 1,2,6, 1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally

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Denne protokol beskriver en undersøgelse af de tidlige interaktioner mellem viralt inficerede nasale epitelceller og medfødt celleaktivering. Individuelle undergrupper af immunceller kan skelnes ud fra deres aktivering som reaktion på virusinfektioner. De kan derefter undersøges yderligere for at bestemme deres virkninger på tidlige antivirale reaktioner.

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Immunologi og infektion udgave 168 Samkultur humane nasale epitelceller medfødte immunceller medfødte T-celler respiratorisk virus influenza cytokiner flowcytometri
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