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Journal / Neuroscience / 一种基于板的测定法，用于测量急性脑切片中内源性单胺释放…
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JoVE Journal Neuroscience
一种基于板的测定法，用于测量急性脑切片中内源性单胺释放
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一种基于板的测定法，用于测量急性脑切片中内源性单胺释放

Article DOI: 10.3791/62127-v • 07:56 min • August 11th, 2021
August 11th, 2021 •
*1, *2, 3, 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine
* These authors contributed equally

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该方法引入了一种使用急性脑切片检测内源性单胺释放的简单技术。该装置使用含有组织支架的48孔板进行单胺释放。释放的单胺通过HPLC结合电化学检测进行分析。此外，该技术还为药物发现提供了一种筛选方法。

Tags

神经科学，第174期，单胺释放，急性脑切片，单胺，多巴胺，去甲肾上腺素，血清素，神经传递
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