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Nachweis von Glykosaminoglykanen durch Polyacrylamid-Gelelektrophorese und Silberfärbung
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Nachweis von Glykosaminoglykanen durch Polyacrylamid-Gelelektrophorese und Silberfärbung

Article DOI: 10.3791/62319-v • 05:57 min • February 25th, 2021
February 25th, 2021 •
1,2, 3,4, 1, 1, 3, 1,5
1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center

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Dieser Bericht beschreibt Techniken zur Isolierung und Reinigung von sulfatierten Glykosaminoglykanen (GAGs) aus biologischen Proben und einen Polyacrylamid-Gelelektrophorese-Ansatz, um ihre Größe anzunähern. GAGs tragen zur Gewebestruktur bei und beeinflussen Signalprozesse durch elektrostatische Interaktion mit Proteinen. Die GAG-Polymerlänge trägt zu ihrer Bindungsaffinität für verwandte Liganden bei.

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Biologie Ausgabe 168
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