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JoVE Journal Bioengineering
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DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Article DOI: 10.3791/62348-v • 06:53 min • March 20th, 2021
March 20th, 2021 •
1, 2, 1, 1, 2, 1,2
1Department of Chemistry, Emory University, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

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This paper describes a detailed protocol for using DNA-based tension probes to image the receptor forces applied by immune cells. This approach can map receptor forces >4.7pN in real-time and can integrate forces over time.

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DNA Tension Probes Molecular Forces Immune Cells Mechanotransduction Mechanobiology Fluorescence Microscopy Force Transmission Coverslips Ethanol Water Ultrasonic Cleaner Piranha Solution Etching
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