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In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

Article DOI: 10.3791/62494-v • 08:15 min • September 8th, 2021
September 8th, 2021 •
1, 1, 1, 1
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

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Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.

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Megakaryopoiesis Transmission Electron Microscopy Ultrastructure Maturation Stages In Situ Exploration Mouse Bone Marrow Native Environment In Vitro Culture Tibia Femurs Razor Blade Epiphyses Removal Cacodylate Buffer 21 Gauge Needle Syringe Bone Marrow Flushing Glutaraldehyde Fixative Solution Agarose Embedding Glass Slide
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