Christian Gachet

Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Affiliated withUniversité de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST Université de Strasbourg, INSERM, EFS Grand Est

Research Area

Biology

Biography

Dr Christian Gachet graduated as a medical doctor in 1985 and gained his Ph.D. in Pharmacology in 1991, both at the University Louis Pasteur, Strasbourg, France. He became a Research Director at INSERM (Institut National de la Santé et de la Recherche Médicale) in 1998. Dr. Gachet is currently the Director of the INSERM Research Unit 1255 and the Director at Etablissement Français du Sang-Grand Est.

The focus of Dr. Gachet work has long been the molecular mechanisms of platelet activation and its inhibition. This led to the characterization and the identification of some of the platelet receptors. He carried out extensive work on the so-called platelet purinergic receptors and the antiplatelet drugs targeting the P2Y12 receptor. Dr Gachet general interest is in platelet physiology (formation of blood platelets, megakaryopoiesis, platelet activation, role of platelets in inflammatory processes and metastatic dissemination) and pharmacology (new drugs, new targets), animal models of platelet defects (BSS, MYH9, P2 receptors), mouse models of arterial thrombosis in a context of atherosclerosis and clinical studies. Since 2012 Dr Gachet is also involved in various topics related to transfusion medicine, including the role of platelets in adverse effects, the impact of pathogen inactivation technologies on platelet functions or blood donation policies. Recent work is focused on production of cultured platelets. Dr Gachet has published over 300 papers in peer-reviewed journals in his area of expertise.

Dr. Gachet has received several distinctions for his work in the field of atherothrombosis. These include the Prix JP Binet in 2004 in France and the Biennial Award for Contributions to Haemostasis, ISTH, 2013, Amsterdam. He has been elected as corresponding member of the French National Academy of Medicine in 2018.

JoVE Journal Publications

ArticleTotal : 7	Year
Publication title Cited by 7	2021
Publication title Cited by 3	2021
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Publication title Cited by 5	2021
Publication title Cited by 3	2021

Other Publications

Article	Year
Platelet δ-Storage Pool Disease: An Update. Journal of clinical medicine\| PubMed ID: 32759727	2020
Disrupting the platelet internal membrane via PI3KC2α inhibition impairs thrombosis independently of canonical platelet activation. Science translational medicine\| PubMed ID: 32718993	2020
Intrinsic impaired proplatelet formation and microtubule coil assembly of megakaryocytes in a mouse model of Bernard-Soulier syndrome. Haematologica\| PubMed ID: 19377075	2009
A central role of GPIb-IX in the procoagulant function of platelets that is independent of the 45-kDa GPIbalpha N-terminal extracellular domain. Blood\| PubMed ID: 20457869	2010
Impact of P2Y12 inhibition by clopidogrel on cardiovascular mortality in unselected patients treated by percutaneous coronary angioplasty: a prospective registry. JACC. Cardiovascular interventions\| PubMed ID: 20630458	2010
Cardiovascular mortality in chronic kidney disease patients undergoing percutaneous coronary intervention is mainly related to impaired P2Y12 inhibition by clopidogrel. Journal of the American College of Cardiology\| PubMed ID: 21251579	2011
Comparison of two murine models of thrombosis induced by atherosclerotic plaque injury. Thrombosis and haemostasis\| PubMed ID: 21479341	2011
The antithrombotic activity of EP224283, a neutralizable dual factor Xa inhibitor/glycoprotein IIbIIIa antagonist, exceeds that of the coadministered parent compounds. The Journal of pharmacology and experimental therapeutics\| PubMed ID: 21527535	2011
Major contribution of the P2Y₁receptor in purinergic regulation of TNFα-induced vascular inflammation. Circulation\| PubMed ID: 21576651	2011
P2 receptors and platelet function. Purinergic signalling\| PubMed ID: 21792575	2011
Romiplostim administration shows reduced megakaryocyte response-capacity and increased myelofibrosis in a mouse model of MYH9-RD. Blood\| PubMed ID: 22234693	2012
Association of estimated GFR with platelet inhibition in patients treated with clopidogrel. American journal of kidney diseases : the official journal of the National Kidney Foundation\| PubMed ID: 22425260	2012
P2Y(12) receptors in platelets and other hematopoietic and non-hematopoietic cells. Purinergic signalling\| PubMed ID: 22528678	2012
The extent of P2Y12 inhibition by clopidogrel in diabetes mellitus patients with acute coronary syndrome is not related to glycaemic control: roles of white blood cell count and body weight. Thrombosis and haemostasis\| PubMed ID: 22740070	2012
Pathogenic and likely pathogenic variants in at least five genes account for approximately 3% of mild isolated nonsyndromic thrombocytopenia. Transfusion\| PubMed ID: 32757236	2020
Purinergic signaling promotes proliferation of adult mouse subventricular zone cells. The Journal of neuroscience : the official journal of the Society for Neuroscience\| PubMed ID: 22764232	2012
Preserved functional and biochemical characteristics of platelet components prepared with amotosalen and ultraviolet A for pathogen inactivation. Transfusion\| PubMed ID: 23067365	2013
Astrocytes protect neurons against methylmercury via ATP/P2Y(1) receptor-mediated pathways in astrocytes. PloS one\| PubMed ID: 23469098	2013
Integrin α6β1 is the main receptor for vascular laminins and plays a role in platelet adhesion, activation, and arterial thrombosis. Circulation\| PubMed ID: 23797810	2013
Involvement of glial P2Y₁ receptors in cognitive deficit after focal cerebral stroke in a rodent model. Journal of neuroinflammation\| PubMed ID: 23890321	2013
Biogenesis of the demarcation membrane system (DMS) in megakaryocytes. Blood\| PubMed ID: 24152908	2014
Contractile forces sustain and polarize hematopoiesis from stem and progenitor cells. Cell stem cell\| PubMed ID: 24268694	2014
FLow-induced PRotrusions (FLIPRs): a platelet-derived platform for the retrieval of microparticles by monocytes and neutrophils. Circulation research\| PubMed ID: 24406984	2014
Proteome changes in platelets after pathogen inactivation--an interlaboratory consensus. Transfusion medicine reviews\| PubMed ID: 24685438	2014
Identification of a new dysfunctional platelet P2Y12 receptor variant associated with bleeding diathesis. Blood\| PubMed ID: 25428217	2015
The P2X1 receptor is required for neutrophil extravasation during lipopolysaccharide-induced lethal endotoxemia in mice. Journal of immunology (Baltimore, Md. : 1950)\| PubMed ID: 25480563	2015
NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation. Purinergic signalling\| PubMed ID: 25504514	2015
The class II PI 3-kinase, PI3KC2α, links platelet internal membrane structure to shear-dependent adhesive function. Nature communications\| PubMed ID: 25779105	2015
Dehydration of blood platelets by zeodration: in vitro characterization and hemostatic properties in vivo. Transfusion\| PubMed ID: 25856501	2015
Resolving the multifaceted mechanisms of the ferric chloride thrombosis model using an interdisciplinary microfluidic approach. Blood\| PubMed ID: 25931587	2015
Blood Cell Palmitoleate-Palmitate Ratio Is an Independent Prognostic Factor for Amyotrophic Lateral Sclerosis. PloS one\| PubMed ID: 26147510	2015
Platelets in cancer. From basic research to therapeutic implications. Hamostaseologie\| PubMed ID: 26289826	2015
Purinergic Receptors in Thrombosis and Inflammation. Arteriosclerosis, thrombosis, and vascular biology\| PubMed ID: 26359511	2015
In Vitro Binding of [³H]PSB-0413 to P2Y₁₂ Receptors. Current protocols in pharmacology\| PubMed ID: 26646192	2015
Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity. PloS one\| PubMed ID: 26808079	2016
[Anti-platelets without a bleeding risk: novel targets and strategies]. Biologie aujourd'hui\| PubMed ID: 26820829	2015
Standardization of a well-controlled in vivo mouse model of thrombus formation induced by mechanical injury. Thrombosis research\| PubMed ID: 26967532	2016
Hemostatic properties and protein expression profile of therapeutic apheresis plasma treated with amotosalen and ultraviolet A for pathogen inactivation. Transfusion\| PubMed ID: 27250038	2016
Coagulation factors bound to procoagulant platelets concentrate in cap structures to promote clotting. Blood\| PubMed ID: 27432876	2016
Inherited dysfunctional platelet P2Y receptor mutations associated with bleeding disorders. Hamostaseologie\| PubMed ID: 27487748	2016
Importance of environmental stiffness for megakaryocyte differentiation and proplatelet formation. Blood\| PubMed ID: 27503502	2016
Respective contributions of single and compound granule fusion to secretion by activated platelets. Blood\| PubMed ID: 27625359	2016
Platelet integrin 61 controls lung metastasis through direct binding to cancer cell-derived ADAM9. JCI insight\| PubMed ID: 27699237	2016
Dual role of IL-21 in megakaryopoiesis and platelet homeostasis. Haematologica\| PubMed ID: 28057742	2017
Macrothrombocytopenia and dense granule deficiency associated with FLI1 variants: ultrastructural and pathogenic features. Haematologica\| PubMed ID: 28255014	2017
Platelet Integrins in Tumor Metastasis: Do They Represent a Therapeutic Target? Cancers\| PubMed ID: 28956830	2017
Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearance. Haematologica\| PubMed ID: 29192131	2017
The role of extracellular matrix stiffness in megakaryocyte and platelet development and function. American journal of hematology\| PubMed ID: 29247535	2018
A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors. Blood advances\| PubMed ID: 29296832	2017
Immobilized fibrinogen activates human platelets through glycoprotein VI. Haematologica\| PubMed ID: 29472360	2018
Imaging thrombosis with Tc-labeled RAM.1-antibody in vivo. Nuclear medicine and biology\| PubMed ID: 29625391	2018
Platelets expressing IgG receptor FcγRIIA/CD32A determine the severity of experimental anaphylaxis. Science immunology\| PubMed ID: 29654057	2018
Platelet transfusion: Current challenges. Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine\| PubMed ID: 30037501	2018
Inherited platelet disorders : Management of the bleeding risk. Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine\| PubMed ID: 30077511	2018
In vitro quality of amotosalen-UVA pathogen-inactivated mini-pool plasma prepared from whole blood stored overnight. Vox sanguinis\| PubMed ID: 30079963	2018
On the way to in vitro platelet production. Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine\| PubMed ID: 30150135	2018
Three-Dimensional Culture in a Methylcellulose-Based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 30171577	2018
High-Resolution 3D Imaging of Megakaryocytes Using Focused Ion Beam-Scanning Electron Microscopy. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 30171581	2018
On the Way to Platelet Production. Frontiers in medicine\| PubMed ID: 30211166	2018
The PI 3-kinase PI3KC2α regulates mouse platelet membrane structure and function independently of membrane lipid composition. FEBS letters\| PubMed ID: 30421419	2019
Clot Contraction Drives the Translocation of Procoagulant Platelets to Thrombus Surface. Arteriosclerosis, thrombosis, and vascular biology\| PubMed ID: 30580561	2019
An essential role for α4A-tubulin in platelet biogenesis. Life science alliance\| PubMed ID: 30760556	2019
The ATP-gated P2X ion channel contributes to the severity of antibody-mediated Transfusion-Related Acute Lung Injury in mice. Scientific reports\| PubMed ID: 30914724	2019
Cell surface expression of HLA I molecules as a marker of young platelets. Journal of thrombosis and haemostasis : JTH\| PubMed ID: 31207003	2019
Combined deficiency of RAB32 and RAB38 in the mouse mimics Hermansky-Pudlak syndrome and critically impairs thrombosis. Blood advances\| PubMed ID: 31399401	2019
Platelet preparation for function testing in the laboratory and clinic: Historical and practical aspects. Research and practice in thrombosis and haemostasis\| PubMed ID: 31624781	2019
Severe bleeding and absent ADP-induced platelet aggregation associated with inherited combined CalDAG-GEFI and P2Y deficiencies. Haematologica\| PubMed ID: 31649128	2020
Use of electron microscopy to study megakaryocytes. Platelets\| PubMed ID: 31903825	2020
High prevalence of the natural Asn89Asp mutation in the GP1BB gene associated with Bernard-Soulier syndrome in French patients from the genetic isolate of Reunion Island. British journal of haematology\| PubMed ID: 31997307	2020
Update of P2Y receptor pharmacology: IUPHAR Review 27. British journal of pharmacology\| PubMed ID: 32037507	2020
Cytoskeletal-based mechanisms differently regulate and proplatelet formation. Haematologica\| PubMed ID: 32327502	2021
Platelet Purinergic Receptors in Thrombosis and Inflammation. Hamostaseologie\| PubMed ID: 32464678	2020
Pharmacological Blockade of Glycoprotein VI Promotes Thrombus Disaggregation in the Absence of Thrombin. Arteriosclerosis, thrombosis, and vascular biology\| PubMed ID: 32698684	2020
Megakaryocytes use in vivo podosome-like structures working collectively to penetrate the endothelial barrier of bone marrow sinusoids. Journal of thrombosis and haemostasis : JTH\| PubMed ID: 32702204	2020
Shear rate gradients promote a bi-phasic thrombus formation on weak adhesive proteins, such as fibrinogen in a VWF-dependent manner. Haematologica\| PubMed ID: 33054087	2020
Differential Role of Glycoprotein VI in Mouse and Human Thrombus Progression and Stability. Thrombosis and haemostasis\| PubMed ID: 33124026	2021
Removal of citrate from PAS-III additive solution improves functional and biochemical characteristics of buffy-coat platelet concentrates stored for 7 days, with or without INTERCEPT pathoge... Transfusion\| PubMed ID: 33527430	2021
Human platelets labeled at two discrete biotin densities are functional in vitro and are detected in vivo in the murine circulation: A promising approach to monitor platelet survival in vivo... Transfusion\| PubMed ID: 33580977	2021
Atypical focal segmental glomerulosclerosis associated with a new PODXL nonsense variant. Molecular genetics & genomic medicine\| PubMed ID: 33780168	2021
CDX2 regulates ACE expression in blood development and leukemia cells. Blood advances\| PubMed ID: 33843985	2021
Correction: An essential role for α4A-tubulin in platelet biogenesis. Life science alliance\| PubMed ID: 34155037	2021
Respective roles of Glycoprotein VI and FcγRIIA in the regulation of αIIbβ3-mediated platelet activation to fibrinogen, thrombus buildup, and stability. Research and practice in thrombosis and haemostasis\| PubMed ID: 34263103	2021
Platelet P2Y Receptor Deletion or Pharmacological Inhibition does not Protect Mice from Sepsis or Septic Shock. TH open : companion journal to thrombosis and haemostasis\| PubMed ID: 34447900	2021