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Beoordeling van wereldwijde DNA double-strand eindresectie met behulp van BrdU-DNA-labeling in combinatie met cell cycle discrimination imaging
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Beoordeling van wereldwijde DNA double-strand eindresectie met behulp van BrdU-DNA-labeling in combinatie met cell cycle discrimination imaging

Article DOI: 10.3791/62553-v • 06:44 min • April 28th, 2021
April 28th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion
* These authors contributed equally

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In dit protocol laten we zien hoe dna dubbelstrengs eindresectie tijdens de S/G2-fase van de celcyclus kan worden gevisualiseerd met behulp van een op immunofluorescentie gebaseerde methode.

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Biologie BrdU foci Immunofluorescentie DNA-eindresectie homologe recombinatie PARP-1 PARP-remmers PARP-remmerresistentie BMN673
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