An Intact Pericardium Ischemic Rodent Model

Ali Fatehi Hassanabad; Darrell D. Belke; Jeannine Turnbull; Jameson A. Dundas; Vishnu Vasanthan; Guoqi Teng; Paul W. M. Fedak; Justin F. Deniset

doi:10.3791/62720

Um modelo de roedor isquêmico de pericárdio intacto

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

An Intact Pericardium Ischemic Rodent Model

Um modelo de roedor isquêmico de pericárdio intacto

Full Text

3,072 Views

07:15 min

September 2, 2021

DOI: 10.3791/62720-v

Ali Fatehi Hassanabad¹, Darrell D. Belke¹, Jeannine Turnbull¹, Jameson A. Dundas¹, Vishnu Vasanthan¹, Guoqi Teng¹, Paul W. M. Fedak¹, Justin F. Deniset^1,2

¹Section of Cardiac Surgery, Department of Cardiac Sciences, Libin Cardiovascular Institute,Cumming School of Medicine, ²Department of Pharmacology and Physiology,University of Calgary

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This protocol outlines the steps for inducing myocardial infarction in mice while preserving the pericardium and its contents. This method allows for the evaluation of the role of the pericardial cavity in cardiac repair following myocardial infarction.

Key Study Components

Area of Science

Cardiology
Neuroscience
Animal Models

Background

Myocardial infarction is a critical condition affecting heart function.
The pericardial cavity plays a significant role in cardiac repair.
Conventional methods often compromise the pericardial cavity.
Understanding the pericardial response can inform therapeutic strategies.

Purpose of Study

To maintain an intact pericardial cavity during myocardial infarction modeling.
To assess the role of pericardial contents in heart healing.
To improve understanding of cardiac injury responses.

Methods Used

Post-anesthetization, the mouse is restrained and positioned for surgery.
Ventilation with 2% isoflurane and 100% oxygen is initiated.
Specific ventilatory settings are applied for the procedure.
Myocardial infarction is induced while preserving the pericardium.

Main Results

The method allows for the study of pericardial contents post-infarction.
Insights into cardiac repair mechanisms are gained.
Potential therapeutic strategies targeting the pericardium are identified.

Conclusions

Maintaining the pericardial cavity enhances understanding of cardiac repair.
This method provides a novel approach to studying myocardial infarction.
Future research can focus on therapeutic interventions involving the pericardium.

Frequently Asked Questions

What is the significance of preserving the pericardium?

Preserving the pericardium allows for a better understanding of its role in cardiac repair following injury.

How does this method differ from conventional techniques?

Unlike conventional methods, this technique maintains an intact pericardial cavity, providing insights into its contents and functions.

What are the implications of this research?

The findings could lead to new therapeutic strategies that target the pericardium to enhance heart healing.

What type of anesthesia is used in this protocol?

The protocol uses 2% isoflurane for anesthesia during the surgical procedure.

What are the ventilatory settings for the mice?

The mice are ventilated at a rate of 110 breaths per minute with a tidal volume of 250 microliters.

Can this method be applied to other animal models?

While this method is designed for mice, adaptations may be possible for other species.

Este protocolo descreve as etapas para induzir o infarto do miocárdio em camundongos, preservando o pericárdio e seu conteúdo.

Este método nos permitirá avaliar o papel da cavidade pericárdica que envolve o coração e seu conteúdo no reparo cardíaco após o infarto do miocárdio. Em comparação com os métodos convencionais de ligadura coronariana para modelagem do infarto do miocárdio, nosso método mantém uma cavidade pericárdica intacta, permitindo a determinação do conteúdo e rastreia seus papéis após a lesão isquêmica cardíaca. Compreender a contribuição da cavidade pericárdica e a resposta à lesão do coração fornecerá informações importantes sobre estratégias terapêuticas que visam o pericárdio para melhorar a cicatrização do coração.

Para começar com a pós-anestesia, restrinja as patas do rato e posicione-o sobre a mesa cirúrgica. Depois de preparar o rato para a cirurgia, conforme descrito no manuscrito, ventilar o rato com 2%isoflurano e 100% de oxigénio como gás transportador utilizando um ventilador comercial. Em seguida, defina uma taxa de 110 respirações por minuto, um volume corrente de 250 microlitros e uma pressão expiratória final positiva de quatro milímetros de mercúrio.

View the full transcript and gain access to thousands of scientific videos