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Kvantitativ analyse af Aspergillus nidulans vækstrate ved hjælp af Live Microscopy og Open Source Software
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Kvantitativ analyse af Aspergillus nidulans vækstrate ved hjælp af Live Microscopy og Open Source Software

Article DOI: 10.3791/62778-v • 11:30 min • July 24th, 2021
July 24th, 2021 •
1,2, 1, 1, 1
1Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications, National Centre for Scientific Research, Demokritos (NCSRD), 2Light Microscopy Unit, Institute of Biosciences and Applications, National Centre for Scientific Research, Demokritos (NCSRD)

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Vi præsenterer en etiketfri live imaging protokol ved hjælp af transmitterede lysmikroskopi teknikker til at fange billeder, analysere og kvantificere vækst kinetik af filamentous svamp A. nidulans i både nedsænket kulturer og faste medier. Denne protokol kan bruges sammen med fluorescensmikroskopi.

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Biologi Udgave 173 Aspergillus vækstrate svampe levende celle billeddannelse ImageJ
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