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Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
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Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Article DOI: 10.3791/62968-v • 09:07 min • September 20th, 2021
September 20th, 2021 •
1, 1, 1, 1
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa

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This protocol presents a new reporter gene system and the experimental setup to detect transcription at DNA double-strand breaks with single-molecule sensitivity.

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Live-cell Imaging Transcriptional Activity DNA Double-strand Breaks Observe Detect Transcription Events RNA Transcripts Time Intervals DNA Damage Response Crosstalk Cellular Processes Doxycycline Calibration Measurements Solution A Solution B Transfection Helper Reagent Lipid-based Transfection Reagent Transfect Cells Glass Bottom Dish
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