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Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
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Biologia
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JoVE Journal Biologia
Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

DOI:
10.3791/63157-v

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05:50 min

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November 01, 2021

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, , , , , ,
1The Azrieli Faculty of Medicine,Bar-Ilan University, 2Department of Molecular Biophysics and Biochemistry,Yale University, 3Department of Neuroscience,Yale University

Capítulos

  • 00:04Introduction
  • 00:47Glass-Bottom Dish Coating
  • 01:48Preparation of Cells for Imaging
  • 02:27Microscope Setup and Imaging
  • 03:23Image Analysis
  • 04:29Results: Cell-Edge Protrusion Assay at the Edge of Spreading Cells
  • 05:08Conclusion

Summary

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This protocol aims to measure the dynamic parameters (protrusions, retractions, ruffles) of protrusions at the edge of spreading cells.

Tags

Cell-edge ProtrusionCell MigrationLive-cell MicroscopyCell MotilityFibronectinBSATrypsinHemacytometerPhase Contrast Microscopy

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