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Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Article DOI: 10.3791/63383-v • 14:51 min • June 17th, 2022
June 17th, 2022 •
1,2, 1,2, 2,3, 2,3, 1,2,3, 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

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High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).

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Pooled ShRNA Library Screening Negative Selection Screening Epigenetic Targets Cytarabine Resistance AML RNAi Screening MV4-11 Cell Line Promoters Lentiviruses Spinfection GFP Quantification
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