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JoVE Journal Bioengineering
Producción automatizada a gran escala de esferoides de células madre derivadas de tejido adiposo para bioimpresión 3D
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Producción automatizada a gran escala de esferoides de células madre derivadas de tejido adiposo para bioimpresión 3D

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Aquí, describimos la producción a gran escala de esferoides estromales /células madre (ASC) derivados de tejido adiposo utilizando un sistema de pipeteo automatizado para sembrar la suspensión celular, asegurando así la homogeneidad del tamaño y la forma del esferoide. Estos esferoides ASC se pueden utilizar como bloques de construcción para enfoques de bioimpresión 3D.

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Bioingeniería Número 181
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