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JoVE Journal Immunology and Infection
Organoides intestinales caninos en un sistema de soporte permeable de doble cámara
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Organoides intestinales caninos en un sistema de soporte permeable de doble cámara

Article DOI: 10.3791/63612-v • 11:11 min • March 2nd, 2022
March 2nd, 2022 •
1, 1, 2, 3, 2, 3, 4, 5, 2, 2,3, 1,3
1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation, Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, Food and Drug Administration

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Aquí, presentamos un protocolo que describe el cultivo de organoides intestinales caninos en un sistema de soporte permeable de doble cámara. Se describe la siembra de organoides en los soportes permeables, el mantenimiento de la monocapa y los experimentos posteriores de permeabilidad al fármaco.

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Inmunología e Infección Número 181 Soporte permeable Cámara dual Canino Organoide Intestino Célula madre Investigación traslacional Investigación traslacional inversa Iniciativa One Health Descubrimiento de fármacos Permeabilidad a fármacos
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