Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Durchflusszytometrische Analyse von Biomarkern…
JoVE Journal
Medicine
Author Produced

Este de .

JoVE Journal Medicine
Durchflusszytometrische Analyse von Biomarkern zum Nachweis von Funktionsdefekten menschlicher Spermien
Assessment of Pulmonary Capillary Blood Volume, Membrane Diffusing Capacity, and Intrapulmonary Arteriovenous Anastomoses During Exercise
Journal (Medicine)
Assessment of Pulmonary Capillary Blood Volume, Membrane Diffusing Capacity, and Intrapulmonary Arteriovenous Anastomoses During Exercise
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
Journal (Medicine)
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
 
Click here for the English version

Durchflusszytometrische Analyse von Biomarkern zum Nachweis von Funktionsdefekten menschlicher Spermien

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Das vorliegende Protokoll bietet eine praktische Lösung, die die Messung von Apoptose, mitochondrialem Membranpotenzial und DNA-Schäden in menschlichen Spermien mit einem einzigen Zytometer ermöglicht.

Tags

Durchflusszytometrie Biomarker Funktionsdefekte der Spermien konventionelle Samenparameter idiopathische Unfruchtbarkeit Spermienapoptose Mitochondrialmembranpotenzial (MMP) DNA-Schäden FCM-Techniken Annexin-V-Fluorescein-Isothiocyanat (FITC)/Propidiumiodid (PI)-Färbung 5,5& 8242;,6,6&8242;-Tetrachlor-1,1&8242;,3,3&8242;-Tetraethyl-benzimidazolylcarbocyanin-Iodid-Sonde (JC-1) Spermienchromatin-Struktur-Assay (SCSA) Acridinorange (AO)-Färbung
Read Article
Waiting X
Simple Hit Counter