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Developmental Biology

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JoVE Journal Developmental Biology
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells
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Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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The present protocol describes human induced pluripotent stem cell-derived type 2 alveolar epithelial-like cells (iAT2s). These cells can be cultured as self-renewing spheres in 3D culture or adapted to air-liquid interface (ALI) culture.

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3D Spheres 2D Air-liquid Interface Cultures Human Induced Pluripotent Stem Cells Type 2 Alveolar Epithelial Cells Culturing Method Passaging Lung Biology Environmental Exposure Pulmonary Diseases Genetic Variants Environmental Exposures Infectious Agents CK DCI Medium Matrix Droplets Alveolospheres Directed Differentiation Dispase P-1000 Pipette Dissociated Organoids Iscove's Modified Dulbecco's Medium Centrifuge Trypsin
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