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JoVE Journal Developmental Biology
Generering av luftvägsepitelcell luft-vätskegränssnittskulturer från mänskliga pluripotenta stamceller
Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
Journal (Developmental Biology)
Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
Journal (Developmental Biology)
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Generering av luftvägsepitelcell luft-vätskegränssnittskulturer från mänskliga pluripotenta stamceller

Article DOI: 10.3791/63882-v • 10:46 min • June 14th, 2022
June 14th, 2022 •
1,2, 2, 3, 2, 2, 3, 1,2, 1,2
1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center

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De senaste framstegen inom humaninducerade pluripotenta stamcellsdifferentieringsprotokoll möjliggör stegvis härledning av organspecifika celltyper. Här ger vi detaljerade steg för underhåll och expansion av iPSC-härledda luftvägsbasalceller och deras differentiering till ett mucociliärt epitel i luft-flytande gränssnittskulturer.

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Utvecklingsbiologi nummer 184
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