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用于使用受激拉曼散射显微镜进行延时活细胞成像的柔性腔室

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

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用于使用受激拉曼散射显微镜进行延时活细胞成像的柔性腔室

DOI:

10.3791/64449-v

•

07:40 min

•

August 31, 2022

•

Yuhao Yuan, Fake Lu

¹Department of Biomedical Engineering, Binghamton University,State University of New York

Capítulos

00:05Introduction
00:48Microscope Environmental Enclosure
01:56Assembly of the Flexible Chamber
03:09Preparation for Time-Lapse Live-Cell SRS Imaging
04:08Time-Lapse Live-Cell SRS Imaging
05:25Results: Time-Lapse SRS Imaging of Live SKOV3 Cells
07:01Conclusion

Summary

Tadução automática

我们报告了一种台顶柔性环境室，用于使用具有透射信号检测的正置受激拉曼散射显微镜对活细胞进行延时成像。脂质液滴在用油酸处理的SKOV3细胞中成像长达24小时，时间间隔为3分钟。

Tags

Flexible Environmental Chamber Time-lapse Live-cell Imaging Stimulated Raman Scattering (SRS) Microscopy Transmitted Signal Detection High Numerical Aperture Objective High-end Condenser Silicone Rubber Sheet Mica Ceramic Sheets Polycarbonate Sheets Flexible Duct Hose Heater Module Machined Aluminum Pieces Natural Rubber Film Sleeve CO2 Cylinder Gas Mixer Module Inline Filters Pre-sterilized Water Bottle Hotplate

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