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デジタル液滴PCRを用いた環状RNAの定量
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デジタル液滴PCRを用いた環状RNAの定量

Article DOI: 10.3791/64464-v • 08:39 min • September 16th, 2022
September 16th, 2022 •
*1,2, *1,2, 1
1Institute of Life Sciences, 2School of Biotechnology, KIIT University
* These authors contributed equally

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このプロトコルでは、ダイバージェントプライマーを使用して細胞内の環状RNA(circRNA)レベルを正確に定量するためのデジタルドロップレットPCR(dd-PCR)の詳細な方法について説明します。

Tags

生物学、第187号、環状RNA、RT-PCR、ダイバージェントプライマー、絶対定量
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