Quantifying Fitness Costs in Transgenic <em>Aedes aegypti</em> Mosquitoes

Adeline E. Williams; Irma Sanchez-Vargas; Lindsay E. Martin; Ines Martin-Martin; Susi Bennett; Ken E. Olson; Eric Calvo

doi:10.3791/65136

Quantificando os custos de aptidão em mosquitos Aedes aegypti transgênicos

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Quantifying Fitness Costs in Transgenic Aedes aegypti Mosquitoes

Quantificando os custos de aptidão em mosquitos Aedes aegypti transgênicos

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09:41 min

September 15, 2023

DOI: 10.3791/65136-v

Adeline E. Williams^1,2, Irma Sanchez-Vargas¹, Lindsay E. Martin^2,3, Ines Martin-Martin^2,4, Susi Bennett¹, Ken E. Olson¹, Eric Calvo²

¹Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology,Colorado State University, ²Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases,National Institutes of Health, ³Department of Biological Sciences,Vanderbilt University, ⁴National Center for Microbiology,Instituto de Salud Carlos III

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Overview

This study presents a detailed protocol for measuring various fitness parameters in Aedes aegypti mosquitoes, crucial for assessing the impact of transgenic modifications. The parameters include fecundity, wing size, fertility, and adult longevity, which serve as indicators of reproductive success.

Key Study Components

Research Area

Transgenic mosquito fitness assessment
Reproductive success
Population dynamics of Aedes aegypti

Background

Importance of fitness metrics in evaluating mosquito populations
Standard measurements commonly used in entomological studies
Significance of Aedes aegypti as a vector for diseases

Methods Used

Egg hatching, larval feeding, and adulthood assessment
Aedes aegypti as the model system
Stereoscopic analysis, genetic crossings, and statistical evaluations

Main Results

Detailed quantification of fecundity, wing size, and other life parameters
Variation in fitness metrics between transgenic and wild type mosquitoes
Clear methodology for tracking mosquito development and reproductive results

Conclusions

The study demonstrates essential techniques for evaluating mosquito fitness, vital for ecological research
Findings contribute to understanding transgenic mosquito impacts on populations

Frequently Asked Questions

What species is the focus of this research?

The research focuses on Aedes aegypti mosquitoes.

Why are fitness parameters important?

They indicate reproductive success and overall population health.

What methods were used to assess mosquito fitness?

Methods include assessing fecundity, wing size, fertility, and longevity of mosquitoes.

How were mating strategies implemented?

Mating involved crossing transgenic males with virgin wild type females in controlled conditions.

What is the significance of wing size measurement?

Wing size is a critical indicator of mosquito fitness and overall health.

Did the study track mortality rates?

Yes, the longevity of male and female mosquitoes was tracked throughout the study.

What implications does this research have?

It helps understand the ecological impact of transgenic mosquitoes on the environment.

O presente protocolo descreve como medir dados de parâmetros de vida comuns em mosquitos Aedes aegypti , incluindo fecundidade, tamanho da asa, fertilidade, proporção sexual, viabilidade, tempos de desenvolvimento, contribuição masculina e longevidade adulta. Essas medições podem ser usadas para avaliar a aptidão de mosquitos transgênicos.

Este protocolo demonstra medidas padrão, refletindo a aptidão, em mosquitos aedes aegypti, esses parâmetros de aptidão foram escolhidos porque refletem o sucesso reprodutivo, são simples de medir e são comumente relatados na literatura. Para começar, coloque papéis de ovo em água estéril durante a noite no setor para chocar mosquitos transgênicos e selvagens. Permita que as larvas se alimentem ad libitum, fornecendo várias gotas de chorume de ração para peixes moídos.

Para garantir que os adultos sejam virgens, separe as pupas por sexo assim que emergirem, mova a pupa para uma lâmina de microscópio de vidro e remova o excesso de água com tecido para imobilizar a pupa. Sob um estereoscópio com ampliação de duas a quatro vezes, usando um pincel de ponta fina, posicione a pupa voltada para cima. Em seguida, analise visualmente a cauda em busca de diferenças na forma do lobo genital.

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Palavras-chave: Aedes aegypti mosquitos aptidão para adultos transgênicos fecundidade papéis de ovos desenvolvimento larval separação de pupas medição de asas