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Journal / Genetics / İnsan Periferik Lenfositlerinde γH2AX ve 53BP1'in …
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Genetics
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JoVE Journal Genetics
İnsan Periferik Lenfositlerinde γH2AX ve 53BP1'in İkili İmmünofloresansı
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İnsan Periferik Lenfositlerinde γH2AX ve 53BP1'in İkili İmmünofloresansı

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Bu protokol, bleomisin ile tedavi edilen insan periferik lenfositlerinin interfaz çekirdeklerinde γH2AX ve 53BP1 odaklarının eşzamanlı tespiti yoluyla DNA çift iplikçik kırılmalarının oluşumunu ve onarımını değerlendirmek için bir yöntem sunmaktadır.

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