Name: Marcação in vivo de isótopos estáveis para identificação por espectrometria de massa de metabólitos paternos transferidos do espermatozoide para o oócito durante a fertilização (Vídeo) | JoVE Journal
Uploaded: 2025-06-17T14:00:00.000+00:00
Duration: 5 min 55 s
Description: Aprenda a marcar isótopos estáveis em espermatozoides de camundongos para rastrear metabólitos paternos transferidos ao oócito durante a fertilização. Este método revela contribuições paternas além do DNA na herança epigenética.

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5 minutes

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<table><tbody><tr><td>2H2O filtrado, 99,8 átomos % 2H</td><td>Sigma Aldrich</td><td>756822</td><td></td></tr><tr><td>Acetona</td><td>Sigma Aldrich</td><td>179124</td><td></td></tr><tr><td>A Agilent&nbsp; SIL-8</td><td>Agilent (Santa Clara, CA, EUA)</td><td></td><td></td></tr><tr><td>C16-Ceramida-13C3-15N</td><td>Padrão LGC</td><td>TRC-C342770-25MG</td><td></td></tr><tr><td>Diclorometano</td><td>Sigma Aldrich</td><td>34856</td><td></td></tr><tr><td>Éter dietílico</td><td>Sigma Aldrich</td><td>673811</td><td></td></tr><tr><td>etanol</td><td>Sigma Aldrich</td><td>1,11,727</td><td></td></tr><tr><td>HCG (gonadotrofina coriônica humana,</td><td>Sigma Aldrich</td><td>CG5-1VL</td><td></td></tr><tr><td>Cloridrato de L-lisina-&épsilon;-15N</td><td>Sigma Aldrich</td><td>608971</td><td></td></tr><tr><td>Masculino e feminino&nbsp; Camundongos C57BL/6JOlaHsd</td><td>Inotiv&nbsp;</td><td>C57BL/6JOlaHsd</td><td></td></tr><tr><td>éter metil terc-butílico (MTBE)</td><td>Sigma Aldrich</td><td>306975</td><td></td></tr><tr><td>MetOH</td><td>Sigma Aldrich</td><td>1,06,035</td><td></td></tr><tr><td>N,O-bis(trimetilsilil(TMS) trifluoroacetamida (BSTFA)</td><td>Supelco</td><td>15238</td><td></td></tr><tr><td>PBS</td><td>Aplicativo</td><td>A0965-9010</td><td></td></tr><tr><td>PMSG (égua grávida' soro)</td><td>Lee Biosoluções</td><td>493-10</td><td></td></tr><tr><td>Piridina</td><td>Sigma Aldrich</td><td>360570</td><td></td></tr><tr><td>Shimadzu GCMS 2010plus&nbsp;</td><td>Shimadzu</td><td></td><td></td></tr><tr><td>Sitostanol-5,6,22,23-d4</td><td>Sigma Aldrich</td><td>680028</td><td></td></tr><tr><td>Trifluoracetato de sódio</td><td>Sigma Aldrich</td><td>1615138</td><td></td></tr><tr><td>SolariX XR 7T&nbsp;</td><td>Bruker Daltonics</td><td></td><td></td></tr><tr><td>TimsTOF Pro Q-TOF&nbsp;</td><td>Bruker Daltonics</td><td></td><td></td></tr><tr><td>Glicose U13C&nbsp;</td><td>Sigma Aldrich</td><td>389374</td><td></td></tr><tr><td>Ultimate RS3000 UHPLC&nbsp;</td><td>Thermo Fisher Científico</td><td></td><td></td></tr><tr><td>Pré-coluna VanGuard CSHTM (5,0 &vezes; 2,1 mm; 1,7 &mu; m, 130 &)</td><td>Águas</td><td></td><td></td></tr></tbody></table>

marcação in vivo de isótopos estáveis para identificação por espectrometria de massa de metabólitos paternos transferidos do espermatozoide para o oócito durante a fertilização

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Marcação in vivo de isótopos estáveis para identificação por espectrometria de massa de metabólitos paternos transferidos do espermatozoide para o oócito durante a fertilização (Vídeo) | JoVE Journal

Marcação in vivo de isótopos estáveis para identificação por espectrometria de massa de metabólitos paternos transferidos do espermatozoide para o oócito durante a fertilização

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