Name: Administração de Δ 9-tetrahidrocanabinol (THC) em camundongos adolescentes e adultos (Vídeo) | JoVE Journal
Uploaded: 2025-08-01T13:40:00.000+00:00
Duration: 7 min 51 s
Description: Aprenda a administrar THC em camundongos adolescentes e adultos para avaliar efeitos farmacodinâmicos agudos. Este protocolo padronizado investiga catalepsia, atividade locomotora e resposta à dor em diferentes estágios de desenvolvimento.

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<li>Renard, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Long-term+consequences+of+adolescent+cannabinoid+exposure+in+adult+psychopathology%5BTitle%5D)+AND+%22Front+Neurosci%22%5BJournal%5D)">Long-term consequences of adolescent cannabinoid exposure in adult psychopathology.</a> Front Neurosci. 8, 361(2014).</li>
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<li>Lee, H. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Frequent+low-dose+delta%289%29-tetrahydrocannabinol+in+adolescence+disrupts+microglia+homeostasis+and+disables+responses+to+microbial+infection+and+social+stress+in+young+adulthood%5BTitle%5D)+AND+%22Biol+Psychiatry%22%5BJournal%5D)">Frequent low-dose delta(9)-tetrahydrocannabinol in adolescence disrupts microglia homeostasis and disables responses to microbial infection and social stress in young adulthood.</a> Biol Psychiatry. 92 (11), 845-860 (2022).</li>
<li>Lim, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adolescent+exposure+to+low-dose+delta9-tetrahydrocannabinol+depletes+the+ovarian+reserve+in+female+mice%5BTitle%5D)+AND+%22Toxicol+Sci%22%5BJournal%5D)">Adolescent exposure to low-dose delta9-tetrahydrocannabinol depletes the ovarian reserve in female mice.</a> Toxicol Sci. 193 (1), 31-47 (2023).</li>
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<li>Fride, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Behavioral+methods+in+cannabinoid+research%5BTitle%5D)+AND+%22Methods+Mol+Med%22%5BJournal%5D)">Behavioral methods in cannabinoid research.</a> Methods Mol Med. 123, 269-290 (2006).</li>
<li>Metna-Laurent, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabinoid-induced+tetrad+in+mice%5BTitle%5D)+AND+%22Curr+Protoc+Neurosci%22%5BJournal%5D)">Cannabinoid-induced tetrad in mice.</a> Curr Protoc Neurosci. 80, 9.59.1-9.59.10 (2017).</li>
<li>Monory, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Genetic+dissection+of+behavioural+and+autonomic+effects+of+delta%289%29-tetrahydrocannabinol+in+mice%5BTitle%5D)+AND+%22PLoS+Biol%22%5BJournal%5D)">Genetic dissection of behavioural and autonomic effects of delta(9)-tetrahydrocannabinol in mice.</a> PLoS Biol. 5 (9), e269(2007).</li>
</ol>

<table><tbody><tr><td>Seringas de 1 mL TB</td><td>BD</td><td>309659</td><td>200/caixa; Estéril, Uso Único.</td></tr><tr><td>Frascos de vidro de 8 mL</td><td>Fisher Scientific&nbsp;</td><td>W224584</td><td>144/Case, usado para preparar a solução de injeção de THC.</td></tr><tr><td>Software de labirinto ANY</td><td>QUALQUER-labirinto</td><td>6000</td><td>Utilizado para gravar vídeos de testes comportamentais e quantificar elementos dos testes para análise de dados.</td></tr><tr><td>Ratos C57BL/6J</td><td>Laboratórios Jackson</td><td>#000664</td><td>Camundongos do tipo selvagem usados para todos os experimentos; Pode especificar a idade exata e o sexo desejados.</td></tr><tr><td>Escala Compass CX</td><td>Ohaus</td><td>CR221</td><td>Usado para pesar ratos para determinar a quantidade de THC a ser injetada; 220 g no máximo, d = 0,1 g.</td></tr><tr><td>Placa Quente Corning Pyroceram</td><td>Fisher Científico</td><td>11500151</td><td>Usado para aquecer banho-maria para o teste Tail Flick.</td></tr><tr><td>Delta-9-tetrahidrocanabinol</td><td>Cayman Chemical&nbsp;</td><td>12068</td><td>Armazenado no cofre trancado com segurança para medicamentos da Tabela 1 em um -30 &ordm; C congelador.</td></tr><tr><td>Luvas de nitrilo Fisherbrand</td><td>Fisher Científico</td><td>19-181-610</td><td>100/pacote; Luvas de uso único, sem látex, usadas para equipamentos de proteção individual adequados.</td></tr><tr><td>GraphPad Prism v10.4</td><td>GraphPad</td><td>n/a</td><td>Software de análise estatística utilizado para analisar dados experimentais e executar testes estatísticos.</td></tr><tr><td>Copo Pesado</td><td>Fisher Científico</td><td>S81280</td><td>Usado para reter água quente para o teste Tail Flick.</td></tr><tr><td>Gás nitrogênio de alta pureza</td><td>Gás de ar</td><td>UN1066</td><td>&nbsp; Usado para evaporar o etanol da solução estoque de THC.</td></tr><tr><td>Logitech C920 Webcamera</td><td>Logitech</td><td>960-001055</td><td>Usado para registrar testes comportamentais em animais; Carl Zeiss 1080p/30 fps HD USB.</td></tr><tr><td>Caixa de campo aberto</td><td>Stoelting Co</td><td>60101</td><td>Caixa com lados opacos para realizar testes de campo aberto; Laterais facilmente removíveis para limpeza.</td></tr><tr><td>Almofadas de treinamento para animais de estimação</td><td>Feliz</td><td>B088G1N2H8</td><td>85/pacote; 22 pol. &agudo; 22 pol.; usado para embrulhar e segurar ratos para o teste Tail Flick.</td></tr><tr><td>Agulhas PrecisionGlide</td><td>BD</td><td>305109</td><td>100/caixa; 0,4 mm e agudo; 13 mm; Estéril, Uso Único.</td></tr><tr><td>Salino</td><td>Fisher Scientific (Cytiva)</td><td>Z1377</td><td>Garrafas de 1 L, grau estéril USP.</td></tr><tr><td>SCOE 1x</td><td>Biofog</td><td>R-SK</td><td>Solução de limpeza usada para limpar materiais de teste comportamental.</td></tr><tr><td>Pré-adolescente-80</td><td>Sigma Aldrich</td><td>P8074-500ML</td><td>BioXtra, líquido viscoso; CAS # 9005-65-6.</td></tr><tr><td>Banho-maria ultrassônico</td><td>Fisher Scientific (Branson)</td><td>CPX2800</td><td>Controle de temperatura; Limpador ultrassônico digital.</td></tr><tr><td>Misturador de vórtice</td><td>Fisher Científico</td><td>2215365</td><td>Usado para misturar o conteúdo da solução de injeção de THC enquanto ela está sendo preparada.</td></tr></tbody></table>

administração de Δ 9-tetrahidrocanabinol (thc) em camundongos adolescentes e adultos

Administration of &#916;9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice

Administração de Δ 9-tetrahidrocanabinol (THC) em camundongos adolescentes e adultos

&#916;9-tetrahydrocannabinol (THC) is responsible for the intoxicating (psychotropic) effects of cannabis and cannabis-derived products currently ...

administration-9-tetrahydrocannabinol-thc-adolescent-adult

Research

JoVE Journal

Neuroscience

Neurociência

A auto-administração intravenosa paradigma (IVSA) é considerado como sendo o padrão de ouro para examinar as propriedades de reforço de drogas de abuso em roedores. Este manuscrito descreve os procedimentos experimentais e técnicas cirúrgicas necessárias para a obtenção de dados confiáveis ​​IVSA. Em particular, a implantação do cateter meticulosa e manutenção são realçados.

Métodos de auto-administração intravenosa em um modelo de camundongo

Este protocolo descreve duas manipulações ambientais diferentes e um protocolo de infusão cerebral concomitante para estudar as alterações cerebrais induzidas pelo ambiente subjacentes ao comportamento adaptativo e ao reparo cerebral em camundongos adultos.

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Este protocolo destina-se a permitir que os pesquisadores conduzam experimentos projetados para testar esses aspectos do vício usando a preferência de lugar condicionada e ensaios de sensibilização comportamental locomotora.

Avaliação da sensibilização comportamental induzida por cocaína e preferência por lugar condicionado em Ratos

O receptor transitório potencial vanilloide tipo 1 (TRPV1) na região supraspinal tem sido sugerido para desempenhar alguns papéis na função cerebral. Descrito aqui é um protocolo para injeção intracerebroventricular de resiniferatoxina para dessensibilização trpv1 supraspinal em camundongos. Procedimentos para alguns exames de dor também são apresentados.

Tratamento intracerebroventricular com resiniferatoxina e testes de dor em camundongos

Aqui nós apresentamos o protocolo imprevisíveis stress leve crônica em ratos. Este protocolo induz um fenótipo de depressivo, como a longo prazo e permite avaliar a eficácia dos antidepressivos putativos em reverter os défices de depressiva como comportamentais e neuromolecular.

O protocolo de estresse suave crônico imprevisível para induzir Anhedonia em ratos

Desenvolvemos um modelo de estresse de derrota social acelerada para camundongos adolescentes C57BL/6, que funciona em machos e fêmeas e permite a exposição durante períodos discretos da adolescência. A exposição a esse modelo induz evitação social, mas apenas em um subconjunto de camundongos machos e fêmeas derrotados.

Modelo de estresse de derrota social para camundongos adolescentes C57BL / 6 machos e fêmeas

Aqui, descrevemos a administração de medicamentos guiada por micropipeta (MDA) como um método alternativo à gavagem oral que incentiva o animal de pesquisa a ingerir tratamentos prontamente com o mínimo de estresse e desconforto.

Uso da administração de medicamentos guiados por micropipeta como método alternativo à gavagem oral em modelos de roedores

Os cartuchos vape destilados de cannabis são dispositivos alimentados por bateria que aerossolizam extratos contendo altas concentrações de canabinóides. A ausência de modelos pré-clínicos estabelecidos para esses produtos cria desafios no estudo de seus efeitos fisiológicos. Para resolver essa lacuna, foi desenvolvido um modelo murino de inalação pré-clínico padronizado para destilados de cannabis vaporizados.

Desenvolvimento de um modelo pré-clínico de inalação para testar destilados de cannabis vaporizados

A administração oral via gavagem em roedores pode induzir sofrimento associado à contenção. Exploramos a administração oromucosa/bucal de extrato de Cannabis enriquecido com CBD, onde os ratos foram tratados por 15 dias com o extrato a 3 mg/kg/dia. Os tratamentos foram administrados por via oral com micropipeta, utilizando-se a técnica de toque afetuoso para manipular os animais.

Oromucosa como método alternativo para administração de produtos de cannabis em roedores

À medida que as restrições legislativas mudam, resultando em maior acessibilidade aos produtos canabinóides, é extremamente importante desenvolver modelos para estudar o impacto dessas exposições, principalmente durante a gravidez. Um modelo pré-clínico de exposição pré-natal moderada a canabinóides por meio de ingestão voluntária foi desenvolvido, permitindo investigações mais aprofundadas.

Modelo pré-clínico de exposição pré-natal ao delta-9-tetrahidrocanabinol para avaliar seu impacto nos resultados do neurodesenvolvimento

Aprenda a administrar THC em camundongos adolescentes e adultos para avaliar efeitos farmacodinâmicos agudos. Este protocolo padronizado investiga catalepsia, atividade locomotora e resposta à dor em diferentes estágios de desenvolvimento.

Administração de Δ 9-tetrahidrocanabinol (THC) em camundongos adolescentes e adultos

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