Name: Ensaio CRISPR/Cas12a de amplificação da polimerase recombinase combinada para detecção de Fusarium oxysporum f. sp. cubense Tropical Race 4 (Vídeo) | JoVE Journal
Uploaded: 2025-11-14T13:35:00.000+00:00
Duration: 6 min 47 s
Description: Aprenda a detectar Fusarium oxysporum f. sp. cubense Tropical Race 4 usando um ensaio CRISPR/Cas12a combinado com amplificação da polimerase recombinase. Este método rápido e econômico melhora a precisão dos testes disponíveis.

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<li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+toxigenic+Fusarium+asiaticum+integrating+recombinase+polymerase+amplification%2C+CRISPR%2FCas12a%2C+and+lateral+flow+techniques%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Rapid and sensitive detection of toxigenic Fusarium asiaticum integrating recombinase polymerase amplification, CRISPR/Cas12a, and lateral flow techniques.</a> Int J Mol Sci. 24 (18), 14134(2023).</li>
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</ol>

<table><tbody><tr><td>Proteína EnGen Lba Cas12A (Cfp1)</td><td>Biolaboratórios da Nova Inglaterra</td><td>M0653T</td><td></td></tr><tr><td>Diluente EnGen Lba Cas12a</td><td>Biolaboratórios da Nova Inglaterra</td><td>B0653A</td><td></td></tr><tr><td>Tubos de PCR Eppendorf</td><td>Merck</td><td>EP0030124332</td><td></td></tr><tr><td>Tubos de Ensaio Micro Eppendorf Safe-Lock</td><td>Merck</td><td>EP0030121708</td><td></td></tr><tr><td>Sacos de Extração Universais</td><td>BIOREBA</td><td>430100</td><td></td></tr><tr><td>GeneRuler Escada de DNA 100 pb</td><td>Thermo Fischer Scientific</td><td>SM0241 / SM0242</td><td></td></tr><tr><td>Centrífuga Genie Mini-6KO</td><td>OptiGene</td><td>OP-FUGE</td><td></td></tr><tr><td>Gênio III</td><td>OptiGene</td><td>https://www.optigene.co.uk/instruments/genie-iii/&nbsp;</td><td></td></tr><tr><td>Suporte para Tira Genie</td><td>OptiGene</td><td>GBLOCK-01 / GBLOCK-02 / GBLOCK-03</td><td></td></tr><tr><td>Tiras Genie</td><td>OptiGene</td><td>OP-0008-50 / OP-0008-500</td><td></td></tr><tr><td>Kit de Síntese de RNA de Alto Rendimento HiScribe T7</td><td>Biolaboratórios da Nova Inglaterra</td><td>E2040S</td><td></td></tr><tr><td>Espectrofotômetro NanoDrop ND-1000 UV/Vis</td><td>Thermo Fischer Scientific</td><td>https://caeonline.com/buy/spectrometers/nanodrop-nd-1000/293669253</td><td></td></tr><tr><td>NEBuffer 3</td><td>Biolaboratórios da Nova Inglaterra</td><td>B7003S</td><td></td></tr><tr><td>NEBuffer r2.1</td><td>Biolaboratórios da Nova Inglaterra</td><td>B6002S</td><td></td></tr><tr><td>Pó de polivinilpirrolidona (PVP)</td><td>Sigma-Aldrich</td><td>9003-39-8</td><td></td></tr><tr><td>Kit de RNA Clean e Concentrador-5</td><td>Zymo Research</td><td>R1013</td><td></td></tr><tr><td>Corante de Carga de RNA</td><td>Thermo Fischer Scientific</td><td>R0641</td><td></td></tr><tr><td>Transiluminador SmartBlue Blue Light</td><td>Instrumentos Accuris</td><td>E4000-E</td><td></td></tr><tr><td>Pellets de hidróxido de sódio (NaOH)</td><td>Merck, EMPARTA</td><td>K277</td><td></td></tr><tr><td>Tris (hidroximetil) aminometano</td><td>Merck</td><td>77-86-1</td><td></td></tr><tr><td>Kit Básico Líquido TwistAmp</td><td>TwistDx</td><td>TALQBAS01</td><td></td></tr></tbody></table>

ensaio crispr/cas12a de amplificação da polimerase recombinase combinada para detecção de fusarium oxysporum f. sp. cubense tropical race 4

Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4

Ensaio CRISPR/Cas12a de amplificação da polimerase recombinase combinada para detecção de Fusarium oxysporum f. sp. cubense Tropical Race 4

Regular and accurate surveillance stands central to the efficient management of plant diseases. It can indicate which course of action is most ...

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Usando o conjunto de DNA, vectores de múltiplos CRISPR podem ser construídos em paralelo numa única reacção de clonagem, tornando a construção de um grande número de vectores CRISPR uma tarefa simples. Tomate raízes peludas são um excelente sistema modelo para validar vetores CRISPR e gerar materiais mutantes.

De alto rendimento CRISPR Vector construção e caracterização de DNA Modificações pela geração de raízes de tomateiro Cabeludo

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Manipulação genética do patógeno de planta<em&gt; Ustilago maydis</em&gt; Estudar Biologia fúngicos e vegetais Microbe Interactions

Aqui, apresentamos um método para engenheiro do genoma de c. elegans usando CRISPR-Cas9 ribonucleoproteínas e modelos de reparo dependente de homologia.

Uma rápida e superficial Pipeline para gerar Genomic ponto mutantes em c. elegans usando CRISPR/Cas9 ribonucleoproteínas

O objetivo deste protocolo é permitir a edição perfeita do genoma C. elegans usando complexos de ribonucleoproteína montados e o marcador co-CRISPR dpy-10 para triagem. Este protocolo pode ser usado para fazer uma variedade de modificações genéticas em C. elegans incluindo inserções, exclusões, substituições genéticas e substituições de codon.

CRISPR/Cas9 Edição do Gene C. elegans rbm-3.2 utilizando o marcador de triagem co-CRISPR dpy-10 e complexos de ribonucleoproteína montados.

Neste trabalho, foi desenvolvido um método de detecção rápido, sensível e portátil de Candidatus Liberibacter asiaticus baseado na amplificação da recombinase polimerase combinada com CRISPR-Cas12a.

Detecção de Candidatus Liberibacter asiaticus implantável em campo usando amplificação de recombinase polimerase combinada com CRISPR-Cas12a

Sistemas CRISPR-Cas e proteínas anti-CRISPR foram integrados ao esquema de portões booleanos em Saccharomyces cerevisiae. Os novos circuitos lógicos pequenos mostraram bom desempenho e aprofundaram a compreensão dos fatores de transcrição baseados em dCas9/dCas12a e das propriedades das proteínas anti-CRISPR.

Circuitos Digitais Gênicos Baseados em Sistemas CRISPR-Cas e Proteínas Anti-CRISPR

Apresentamos um protocolo que combina amplificação da polimerase recombinase com um sistema CRISPR/Cas12a para detecção de traços de vírus de DNA e constrói microscopia portátil para smartphone com uma classificação assistida por inteligência artificial para detecção de vírus de DNA no local de atendimento.

Sistema de detecção de vírus de DNA baseado em RPA-CRISPR/Cas12a-SPM e aprendizado profundo

Aqui, apresentamos a preparação passo a passo de amplificação isotérmica baseada em recombinase liofilizada pré-misturada e reações baseadas em Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), que podem ser usadas para a detecção de biomarcadores de ácido nucleico de patógenos de doenças infecciosas ou outros marcadores genéticos de interesse.

Diagnóstico baseado em CRISPR no local de atendimento com reagentes pré-misturados e liofilizados

Para facilitar a detecção rápida e precisa de Acinetobacter baumannii, apresentamos um protocolo que emprega a amplificação da polimerase recombinase (RPA) em conjunto com a endonuclease LbaCas12a para identificar infecções por A. baumannii .

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Aqui, descrevemos um procedimento simples e rápido para detectar DNA de patógenos de abelhas, como Lotmaria passim e Nosema ceranae, usando uma lise celular pronta para amplificação, amplificação da polimerase recombinase e ensaios CRISPR/Cas12a.

Detecção de DNA no local de parasitas tripanossomatídeos e Nosema ceranae por meio de lise alcalina acoplada ao sistema RPA/CRISPR/Cas12a

Aprenda a detectar Fusarium oxysporum f. sp. cubense Tropical Race 4 usando um ensaio CRISPR/Cas12a combinado com amplificação da polimerase recombinase. Este método rápido e econômico melhora a precisão dos testes disponíveis.

Ensaio CRISPR/Cas12a de amplificação da polimerase recombinase combinada para detecção de Fusarium oxysporum f. sp. cubense Tropical Race 4 (Vídeo) | JoVE Journal

Ensaio CRISPR/Cas12a de amplificação da polimerase recombinase combinada para detecção de Fusarium oxysporum f. sp. cubense Tropical Race 4

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