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JoVE Encyclopedia of Experiments

Biological Techniques

Video encyclopedias of advanced research experiments for scientists in academia and biotech

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272 Videos

Filmed at top university labs

Practice and theory for each experiment

Genome Editing Techniques

16 Videos · 74 Minutes

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

3.5K views

All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System

All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System

7.4K views

Zinc Finger Nuclease-Based Genome Editing: A Technique for Modifying Genome in Human Pluripotent Stem Cells by Double-Stranded Homology Dependant Repair Mechanism

Zinc Finger Nuclease-Based Genome Editing: A Technique for Modifying Genome in Human Pluripotent Stem Cells by Double-Stranded Homology Dependant Repair Mechanism

3.2K views

TALEN-Mediated Targeted Gene Integration: Using Engineered Sequence-Specific Nucleases for the Precise Insertion of Fluorescent Protein Gene into hiPSCs

TALEN-Mediated Targeted Gene Integration: Using Engineered Sequence-Specific Nucleases for the Precise Insertion of Fluorescent Protein Gene into hiPSCs

2.7K views

phiC31-Integrase-Mediated Site-Directed Transgene Integration: A Microinjection Technique for Site-Specific Transgene Integration into an <em>Anopheles</em> Vector

phiC31-Integrase-Mediated Site-Directed Transgene Integration: A Microinjection Technique for Site-Specific Transgene Integration into an <em>Anopheles</em> Vector

2.5K views

Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion

Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion

3K views

Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos

Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos

3.9K views

PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System

PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System

2.9K views

CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic <em>Leptospira</em>

CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic <em>Leptospira</em>

2.8K views

CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model

CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model

4.2K views

CRISPR Concatemer-Mediated Multiple Gene Knockout: A Technique to Simultaneously Knockout Multiple Genes by Non-Homologous End-Joining Pathway in Mouse Intestinal Cells

CRISPR Concatemer-Mediated Multiple Gene Knockout: A Technique to Simultaneously Knockout Multiple Genes by Non-Homologous End-Joining Pathway in Mouse Intestinal Cells

3K views

Short Hairpin RNA-Mediated Gene Knockdown in iHSPCs In Vitro: A Lentivirus-Based shRNA Expression System Delivery into iHSPCs for Knockdown of Specific Gene Expression

Short Hairpin RNA-Mediated Gene Knockdown in iHSPCs In Vitro: A Lentivirus-Based shRNA Expression System Delivery into iHSPCs for Knockdown of Specific Gene Expression

2.7K views

Inducible Tet-On Regulatable System-Based Gene Expression In Vivo: A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model

Inducible Tet-On Regulatable System-Based Gene Expression In Vivo: A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model

4K views

siRNA Reverse Transfection-Based Gene Silencing In Vitro: A Procedure to Deliver Small Interfering RNA in Cultured Cells to Inactivate Target Gene Expression

siRNA Reverse Transfection-Based Gene Silencing In Vitro: A Procedure to Deliver Small Interfering RNA in Cultured Cells to Inactivate Target Gene Expression

3K views

Inducible Tet-Off Regulatable System: An In Vitro Method to Modulate Gene Expression in Cultured Cells Using Tetracycline-Off System

Inducible Tet-Off Regulatable System: An In Vitro Method to Modulate Gene Expression in Cultured Cells Using Tetracycline-Off System

2.5K views

Morpholino Mediated Gene Editing: A Gene Knockdown Technique Using Translational Blocking Morpholino Oligonucleotides in Single-Celled Cavefish Eggs

Morpholino Mediated Gene Editing: A Gene Knockdown Technique Using Translational Blocking Morpholino Oligonucleotides in Single-Celled Cavefish Eggs

2.5K views

Gene Transfer Techniques

14 Videos · 53 Minutes

Bead Loading to Introduce Nucleic Acids Into Adherent Cultured Cells: A Technique to Load Fluorescent Protein-Encoding Plasmids Into Adherent Mammalian Cells

Bead Loading to Introduce Nucleic Acids Into Adherent Cultured Cells: A Technique to Load Fluorescent Protein-Encoding Plasmids Into Adherent Mammalian Cells

3.2K views

Mosquito Embryo Microinjection: A Technique to Deliver Exogenous DNA into Embryo Yolk of <em>Anopheles Gambiae</em> to Generate Germline Mutations

Mosquito Embryo Microinjection: A Technique to Deliver Exogenous DNA into Embryo Yolk of <em>Anopheles Gambiae</em> to Generate Germline Mutations

3K views

Image-Guided Robotic Cell Microinjection: An Automated High-Throughput Technique for the Precise Delivery of Fluorescently-Labeled Polysaccharides Into Single Neuronal Cells in Organotypic Brain Slice Cultures

Image-Guided Robotic Cell Microinjection: An Automated High-Throughput Technique for the Precise Delivery of Fluorescently-Labeled Polysaccharides Into Single Neuronal Cells in Organotypic Brain Slice Cultures

3K views

Hydrodynamic Gene Delivery: A Technique for <em>In Vivo </em>Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection

Hydrodynamic Gene Delivery: A Technique for <em>In Vivo </em>Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection

4.6K views

<em>Agrobacterium</em>-Mediated Genetic Transformation: A Method to Genetically Transform the Rice Genome via Genetically Engineered <em>Agrobacterium tumefaciens</em>

<em>Agrobacterium</em>-Mediated Genetic Transformation: A Method to Genetically Transform the Rice Genome via Genetically Engineered <em>Agrobacterium tumefaciens</em>

4.4K views

Electroporation-Mediated <em>In Vitro</em> Gene Delivery: An Electric Pulse Technique to Introduce Fluorescent Reporter Protein-Encoding Plasmids Into Cultured Cells

Electroporation-Mediated <em>In Vitro</em> Gene Delivery: An Electric Pulse Technique to Introduce Fluorescent Reporter Protein-Encoding Plasmids Into Cultured Cells

2.7K views

Nucleofection of Parasite Forms: An Electroporation-Based Transfection Method to Deliver Fluorescent Protein Encoding-Plasmids Into Sporozoite Nucleus

Nucleofection of Parasite Forms: An Electroporation-Based Transfection Method to Deliver Fluorescent Protein Encoding-Plasmids Into Sporozoite Nucleus

2.5K views

Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun

Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun

2.8K views

Magnetofection-Based Transfection In Vitro: A Magnetic Field-Assisted Technique to Deliver Plasmids Into Primary Mouse Neuronal Cells Using Magnetic Nanoparticles

Magnetofection-Based Transfection In Vitro: A Magnetic Field-Assisted Technique to Deliver Plasmids Into Primary Mouse Neuronal Cells Using Magnetic Nanoparticles

2.5K views

Recombinant Adeno Associated Virus Based Gene Delivery: A Technique for Transgene Delivery and Cardiac-Specific Expression in Rodent Model

Recombinant Adeno Associated Virus Based Gene Delivery: A Technique for Transgene Delivery and Cardiac-Specific Expression in Rodent Model

2.5K views

Nanoblade-Based Delivery of Nucleic Acid Cargo: A Technique to Deliver Cas9-sgRNA Complex to Target Cells via Virus-Like Particles

Nanoblade-Based Delivery of Nucleic Acid Cargo: A Technique to Deliver Cas9-sgRNA Complex to Target Cells via Virus-Like Particles

2.6K views

Lentiviral Vector-Based Perivitelline Injection: A Method to Transduce the Gene of Interest Into Fertilized Single-Cell Mouse Oocytes

Lentiviral Vector-Based Perivitelline Injection: A Method to Transduce the Gene of Interest Into Fertilized Single-Cell Mouse Oocytes

2.5K views

Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery

Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery

2.2K views

Retroviral Mediated Gene Transduction: A Gene Transfer Technique to Deliver a Transgene in Cultured Cells Using Engineered Retroviral Vectors

Retroviral Mediated Gene Transduction: A Gene Transfer Technique to Deliver a Transgene in Cultured Cells Using Engineered Retroviral Vectors

2.7K views

Staining Techniques

30 Videos · 101 Minutes

Oil Red O Staining: A Technique for Staining Neutral Lipids in Hepatocytes for Detecting Hepatic Steatosis In Vitro

Oil Red O Staining: A Technique for Staining Neutral Lipids in Hepatocytes for Detecting Hepatic Steatosis In Vitro

7.3K views

Capsule-Based Negative Staining of Virus Samples on TEM Grids: A Heavy Metal Staining Technique to Visualize the Ultrastructure of Enveloped Viruses

Capsule-Based Negative Staining of Virus Samples on TEM Grids: A Heavy Metal Staining Technique to Visualize the Ultrastructure of Enveloped Viruses

2.6K views

Fluorescent Silver Staining: A Technique to Visualize Total Protein in Polyacrylamide Gels

Fluorescent Silver Staining: A Technique to Visualize Total Protein in Polyacrylamide Gels

2.5K views

Tartrate-Resistant Acid Phosphatase Staining: An <em>In Vitro</em> Technique to Detect TRAP Enzyme-Containing Cultured Osteoclasts

Tartrate-Resistant Acid Phosphatase Staining: An <em>In Vitro</em> Technique to Detect TRAP Enzyme-Containing Cultured Osteoclasts

5.9K views

Silver Staining of Sulfated Glycosaminoglycans: A Staining Method for Visualization of Sulfated Glycosaminoglycans in Polyacrylamide Gels

Silver Staining of Sulfated Glycosaminoglycans: A Staining Method for Visualization of Sulfated Glycosaminoglycans in Polyacrylamide Gels

2.3K views

Picrosirius Red Polarization Staining: A Technique To Detect Fibrosis in Cardiac Tissue Sections Using Bright-Field Microscopy

Picrosirius Red Polarization Staining: A Technique To Detect Fibrosis in Cardiac Tissue Sections Using Bright-Field Microscopy

3.2K views

Wiesner Staining for Visualizing Lignin: A Technique to Stain Lignin Deposits Present in Stems of <em>Arabidopsis Thaliana</em>

Wiesner Staining for Visualizing Lignin: A Technique to Stain Lignin Deposits Present in Stems of <em>Arabidopsis Thaliana</em>

3.8K views

Maule Staining for Lignin: A Technique to Characterize Lignin Distribution in <em>Arabidopsis Thaliana</em> Stem Sections

Maule Staining for Lignin: A Technique to Characterize Lignin Distribution in <em>Arabidopsis Thaliana</em> Stem Sections

3K views

Congo Red Staining: A Technique to Visualize Cellulose Deposits in Plant Stem Sections

Congo Red Staining: A Technique to Visualize Cellulose Deposits in Plant Stem Sections

4K views

Calcofluor White Staining: A Simple Method for UV-Induced Fluorescence Visualization of Cellulose Deposits in Cell Walls of Dicot Plant Stem Sections

Calcofluor White Staining: A Simple Method for UV-Induced Fluorescence Visualization of Cellulose Deposits in Cell Walls of Dicot Plant Stem Sections

4.5K views

Nile Red Staining: A Technique to Detect and Quantify Intracellular Neutral Lipids in Algae

Nile Red Staining: A Technique to Detect and Quantify Intracellular Neutral Lipids in Algae

3.1K views

Von Kossa Staining of Aortic Vascular Smooth Muscle Cells: An In Vitro Technique for Assessment of Arterial Medial Layer Calcification

Von Kossa Staining of Aortic Vascular Smooth Muscle Cells: An In Vitro Technique for Assessment of Arterial Medial Layer Calcification

3K views

Methylene Blue Staining to Assess Metastatic Colony Formation In Vitro: A Staining Procedure to Assess Metastasis by Quantifying Harvested Cancer Cells From Cancer Mouse Model

Methylene Blue Staining to Assess Metastatic Colony Formation In Vitro: A Staining Procedure to Assess Metastasis by Quantifying Harvested Cancer Cells From Cancer Mouse Model

2.5K views

Periodic Acid-Schiff Staining of Cells: An In Vitro Technique to Detect Glycogen Levels in Peripheral Blood Mononuclear Cells

Periodic Acid-Schiff Staining of Cells: An In Vitro Technique to Detect Glycogen Levels in Peripheral Blood Mononuclear Cells

2.8K views

Crystal Violet Staining: A Technique to Visualize Drug-Resistant Cancer Cells Using Colony Formation Assays

Crystal Violet Staining: A Technique to Visualize Drug-Resistant Cancer Cells Using Colony Formation Assays

9.7K views

Lipid Droplet Staining of Larval <em>Drosophila</em> Oenocytes: A Technique for Assessment of Lipid Droplet Accumulation Under Normal and Stressed Condition Using BODIPY-Based Dye

Lipid Droplet Staining of Larval <em>Drosophila</em> Oenocytes: A Technique for Assessment of Lipid Droplet Accumulation Under Normal and Stressed Condition Using BODIPY-Based Dye

2.6K views

Alizarin Red Staining: A Technique to Visualize Mineralization by Cultured Osteoblasts

Alizarin Red Staining: A Technique to Visualize Mineralization by Cultured Osteoblasts

4.4K views

Giemsa Staining for Colony Formation Assay: An In Vitro Staining Procedure to Assess Proliferative Capability of Mesenchymal Stem Cells

Giemsa Staining for Colony Formation Assay: An In Vitro Staining Procedure to Assess Proliferative Capability of Mesenchymal Stem Cells

3.5K views

Alcian Blue Staining to Visualize Proteoglycans: A Technique to Detect Proteoglycans in Mesenchymal Stem Cell Differentiated Chondrocytes Culture <em>In Vitro</em>

Alcian Blue Staining to Visualize Proteoglycans: A Technique to Detect Proteoglycans in Mesenchymal Stem Cell Differentiated Chondrocytes Culture <em>In Vitro</em>

3.5K views

Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

2.6K views

Double-Staining Method: A Technique to Visualize Plant-Fungus Interactions Using Ruthenium Red and Lactophenol Cotton Blue

Double-Staining Method: A Technique to Visualize Plant-Fungus Interactions Using Ruthenium Red and Lactophenol Cotton Blue

2.9K views

Eosin Staining of Soft-Tissue Samples: An Eosin-Based Procedure for Whole Mouse Kidney Staining to Visualize Specific Tissue Microstructures

Eosin Staining of Soft-Tissue Samples: An Eosin-Based Procedure for Whole Mouse Kidney Staining to Visualize Specific Tissue Microstructures

2.6K views

H&E Staining of Paraffin-Embedded Tissue Sections: A Differential Staining Technique to Visualize Liver Tissue Sections Using Combination of Hematoxylin and Eosin Dyes

H&E Staining of Paraffin-Embedded Tissue Sections: A Differential Staining Technique to Visualize Liver Tissue Sections Using Combination of Hematoxylin and Eosin Dyes

8.6K views

Safranin-O Staining: A Method to Visualize the Structural Integrity and Cellularity of Cartilage

Safranin-O Staining: A Method to Visualize the Structural Integrity and Cellularity of Cartilage

4K views

Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization

Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization

3.5K views

Cresyl Violet Staining of Tissue Sections: A Staining Procedure to Visualize Cartilage and Bones in Frozen Mouse Embryo Sections

Cresyl Violet Staining of Tissue Sections: A Staining Procedure to Visualize Cartilage and Bones in Frozen Mouse Embryo Sections

2.7K views

hFTAA Staining of Amyloid Deposits: A Technique to Visualize Amyloid Fibrils Using Oligothiophene-Based Dye for Fluorescence Imaging in Tissue Sections

hFTAA Staining of Amyloid Deposits: A Technique to Visualize Amyloid Fibrils Using Oligothiophene-Based Dye for Fluorescence Imaging in Tissue Sections

2.5K views

TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury

TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury

5.5K views

Masson Goldner Trichrome Staining: A Technique to Visualize Collagen in Heart Sections for Cardiovascular Fibrosis Detection

Masson Goldner Trichrome Staining: A Technique to Visualize Collagen in Heart Sections for Cardiovascular Fibrosis Detection

3.5K views

Sudan IV Staining: A Procedure for Staining Lipid-Laden Atherosclerotic Plaques in Harvested Mouse Aortic Arch

Sudan IV Staining: A Procedure for Staining Lipid-Laden Atherosclerotic Plaques in Harvested Mouse Aortic Arch

4.1K views

Chromatography Techniques

15 Videos · 58 Minutes

Anion-Exchange Chromatography-Based Protein Purification: A Separation Technique to Isolate a Protein of Interest From Dialyzed Bacterial Lysate Based on Net Charge

Anion-Exchange Chromatography-Based Protein Purification: A Separation Technique to Isolate a Protein of Interest From Dialyzed Bacterial Lysate Based on Net Charge

2.3K views

Calcium-Dependent Hydrophobic Interaction Chromatography: A Technique to Purify Calcium-Binding Proteins Based on Hydrophobic Interactions

Calcium-Dependent Hydrophobic Interaction Chromatography: A Technique to Purify Calcium-Binding Proteins Based on Hydrophobic Interactions

1.4K views

Nickel Affinity Chromatography-Based Protein Purification: A Technique to Purify Polyhistidine-Tagged Recombinant Proteins from Bacterial Cell Lysate

Nickel Affinity Chromatography-Based Protein Purification: A Technique to Purify Polyhistidine-Tagged Recombinant Proteins from Bacterial Cell Lysate

5.9K views

Thin Layer Chromatography-Based Separation of Mycolic Acid Variants: A Method to Separate Mycobacterial Cell Wall Lipids Based on Polarity

Thin Layer Chromatography-Based Separation of Mycolic Acid Variants: A Method to Separate Mycobacterial Cell Wall Lipids Based on Polarity

1.4K views

Heparin Affinity Chromatography-Based Baculovirus Purification: A Technique to Isolate Baculovirus From Insect Cell Supernatant

Heparin Affinity Chromatography-Based Baculovirus Purification: A Technique to Isolate Baculovirus From Insect Cell Supernatant

1.4K views

Cation Exchange Chromatography: A Technique to Isolate Target Recombinant Protein from Insect Cell Lysate Based on Net Surface Charge

Cation Exchange Chromatography: A Technique to Isolate Target Recombinant Protein from Insect Cell Lysate Based on Net Surface Charge

1.4K views

Hydrophilic Interaction Liquid Chromatography: A Technique to Separate Hydrophilic Polar Analytes Using Hydrophilic Beads

Hydrophilic Interaction Liquid Chromatography: A Technique to Separate Hydrophilic Polar Analytes Using Hydrophilic Beads

1.5K views

Size Exclusion Chromatography Based Separation of Bacterial Extracellular Vesicles: A Technique to Separate Heterogenous Population of Gram-Negative Bacterial Outer Membrane Vesicles Based on Their Size

Size Exclusion Chromatography Based Separation of Bacterial Extracellular Vesicles: A Technique to Separate Heterogenous Population of Gram-Negative Bacterial Outer Membrane Vesicles Based on Their Size

1.5K views

Butyl-Dependant Hydrophobic Interaction Liquid Chromatography: A Technique to Characterize Antibody-Drug Conjugates Based on Hydrophobic Interactions

Butyl-Dependant Hydrophobic Interaction Liquid Chromatography: A Technique to Characterize Antibody-Drug Conjugates Based on Hydrophobic Interactions

1.2K views

Reversed-Phase High-Performance Liquid Chromatography: A Robust Method for Quantitation of Fluorescently Labeled and Derivatized Sialic Acids Isolated from Mouse Liver

Reversed-Phase High-Performance Liquid Chromatography: A Robust Method for Quantitation of Fluorescently Labeled and Derivatized Sialic Acids Isolated from Mouse Liver

1.3K views

Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

1.4K views

DFE-Based Affinity Chromatography: A Technique for Purification of 2F5 Monoclonal Antibodies from a Crude Sample Using High Ionic Strength-Based Elution Conditions

DFE-Based Affinity Chromatography: A Technique for Purification of 2F5 Monoclonal Antibodies from a Crude Sample Using High Ionic Strength-Based Elution Conditions

1.3K views

Thin Layer Chromatography Based Enzyme Activity Assay: A Technique to Determine In Vitro Pyrophosphokinase Activity Using Thin Layer Chromatography

Thin Layer Chromatography Based Enzyme Activity Assay: A Technique to Determine In Vitro Pyrophosphokinase Activity Using Thin Layer Chromatography

1.6K views

Headspace Gas Chromatography: A Technique to Assess Ethanol Concentration in Zebrafish Embryos

Headspace Gas Chromatography: A Technique to Assess Ethanol Concentration in Zebrafish Embryos

1.4K views

High-Performance Thin Layer Chromatography: A Technique for Preliminary Separation and Quantification of Lipids From Neutrophils

High-Performance Thin Layer Chromatography: A Technique for Preliminary Separation and Quantification of Lipids From Neutrophils

1.7K views

Electrophoresis Techniques

15 Videos · 66 Minutes

Polyacrylamide Gel Electrophoresis: An Analytical Technique to Detect Heparan Sulfates of Varying Chain Lengths Isolated from Mouse Lung Tissue

Polyacrylamide Gel Electrophoresis: An Analytical Technique to Detect Heparan Sulfates of Varying Chain Lengths Isolated from Mouse Lung Tissue

2.6K views

Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis for Protein Analysis: A Technique to Separate and Visualize Proteins Based on Molecular Weight

Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis for Protein Analysis: A Technique to Separate and Visualize Proteins Based on Molecular Weight

2.5K views

Denaturing Urea Polyacrylamide Gel Electrophoresis for RNA Analysis: A Technique to Separate Fluorescently Labeled Phosphorylated RNA Oligonucleotides from their Non-Phosphorylated Equivalents

Denaturing Urea Polyacrylamide Gel Electrophoresis for RNA Analysis: A Technique to Separate Fluorescently Labeled Phosphorylated RNA Oligonucleotides from their Non-Phosphorylated Equivalents

2.1K views

Two-Dimensional Semi-Denaturing Agarose Gel Electrophoresis: A Technique to Separate Polymorphic Amyloids Fibers Based on Size Heterogeneity

Two-Dimensional Semi-Denaturing Agarose Gel Electrophoresis: A Technique to Separate Polymorphic Amyloids Fibers Based on Size Heterogeneity

1.4K views

Blue Native Polyacrylamide Gel Electrophoresis: A Non-Denaturing Separation Technique for Analysis of Intact Mitochondrial Respiratory Chain Complexes

Blue Native Polyacrylamide Gel Electrophoresis: A Non-Denaturing Separation Technique for Analysis of Intact Mitochondrial Respiratory Chain Complexes

2.1K views

Multimer-PAGE for Separating Native Protein Complexes: A Hybrid Separation Technique Consisting of Blue Native-PAGE and SDS-PAGE to Separate Intact Multimeric Proteins From Tissue Lysate

Multimer-PAGE for Separating Native Protein Complexes: A Hybrid Separation Technique Consisting of Blue Native-PAGE and SDS-PAGE to Separate Intact Multimeric Proteins From Tissue Lysate

1.9K views

Agarose Gel Electrophoresis of DNA Amplicons Post PCR: A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success

Agarose Gel Electrophoresis of DNA Amplicons Post PCR: A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success

4.6K views

Capillary Electrophoresis: A Technique to Analyze Fluorescently Labeled Polymerase Chain Reaction Amplicons

Capillary Electrophoresis: A Technique to Analyze Fluorescently Labeled Polymerase Chain Reaction Amplicons

1.6K views

Alkaline Single Cell Gel Electrophoresis: A Sensitive Technique for Quantitative Estimation of DNA Damage in Individual Cancer Cells Following Chemotherapy

Alkaline Single Cell Gel Electrophoresis: A Sensitive Technique for Quantitative Estimation of DNA Damage in Individual Cancer Cells Following Chemotherapy

1.4K views

Microfluidic Capillary Electrophoresis for Fragment Sizing of PCR Products: A Microchip-Based Electrophoretic Separation Technique to Separate DNA Fragments Based on Size

Microfluidic Capillary Electrophoresis for Fragment Sizing of PCR Products: A Microchip-Based Electrophoretic Separation Technique to Separate DNA Fragments Based on Size

1.6K views

Non-Reducing SDS PAGE: A Method to Analyze Disulfide-Linked Multimeric Protein Complexes

Non-Reducing SDS PAGE: A Method to Analyze Disulfide-Linked Multimeric Protein Complexes

6.4K views

Liquid Phase Isoelectric Focusing: An Electrophoresis Technique to Separate Bioactive Molecules from Crude <em>Gymnema sylvestre</em> Extract

Liquid Phase Isoelectric Focusing: An Electrophoresis Technique to Separate Bioactive Molecules from Crude <em>Gymnema sylvestre</em> Extract

1.3K views

Mobility Shift Affinity Capillary Electrophoresis: A Method to Analyze Sample-Ligand Interactions Depending on Differential Migration of Protein-Ligand Complexes

Mobility Shift Affinity Capillary Electrophoresis: A Method to Analyze Sample-Ligand Interactions Depending on Differential Migration of Protein-Ligand Complexes

1.4K views

Two-Dimensional Polyacrylamide Gel Electrophoresis: A Separation Technique for Analysis of Proteins in Complex Protein Mixture Based on Charge and Molecular Weight

Two-Dimensional Polyacrylamide Gel Electrophoresis: A Separation Technique for Analysis of Proteins in Complex Protein Mixture Based on Charge and Molecular Weight

1.8K views

Zymography Gel Electrophoresis: An Electrophoretic Technique to Detect Matrix Metalloproteinases by Assessing the Enzymatic Activity

Zymography Gel Electrophoresis: An Electrophoretic Technique to Detect Matrix Metalloproteinases by Assessing the Enzymatic Activity

1.7K views

Assay Techniques

102 Videos · 421 Minutes

Phospholipase Activity Assay: A Gel Diffusion Assay to Determine Phospholipase Activity in Crude Venom Extract from <em> Anthopleura dowii </em>

Phospholipase Activity Assay: A Gel Diffusion Assay to Determine Phospholipase Activity in Crude Venom Extract from <em> Anthopleura dowii </em>

920 views

Ring-Shaped Micropatterning Cell Chirality Assay: An In Vitro Technique to Determine Multicellular Chirality Based on Cell Alignment on Ring-Shaped Micropatterns

Ring-Shaped Micropatterning Cell Chirality Assay: An In Vitro Technique to Determine Multicellular Chirality Based on Cell Alignment on Ring-Shaped Micropatterns

464 views

Citrate Synthase Activity Assay: A Colorimetric Assay to Measure Mitochondrial Citrate Synthase Activity in Drosophila Tissue Homogenate

Citrate Synthase Activity Assay: A Colorimetric Assay to Measure Mitochondrial Citrate Synthase Activity in Drosophila Tissue Homogenate

794 views

Bathophenanthroline Sulfonate-Based Colorimetric Assay: A Simple and Rapid Method for Quantitation of Non-Heme Iron in Mouse Liver Tissue

Bathophenanthroline Sulfonate-Based Colorimetric Assay: A Simple and Rapid Method for Quantitation of Non-Heme Iron in Mouse Liver Tissue

689 views

Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO<sub>2</sub>

Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO<sub>2</sub>

474 views

Planar Supported Lipid Bilayer Assay: A Microscopy-Based In Vitro Technique to Investigate Septin Assembly on Biological Membrane Mimics

Planar Supported Lipid Bilayer Assay: A Microscopy-Based In Vitro Technique to Investigate Septin Assembly on Biological Membrane Mimics

585 views

Spherical Supported Lipid Bilayer Assay: A Technique to Observe the Septin Assembly on Microspheres

Spherical Supported Lipid Bilayer Assay: A Technique to Observe the Septin Assembly on Microspheres

432 views

Small Molecule Nematicides Screening Assay: A Medium Throughput Method to Screen Potential Nematicides Against <em> Ditylenchus dipsaci </em>

Small Molecule Nematicides Screening Assay: A Medium Throughput Method to Screen Potential Nematicides Against <em> Ditylenchus dipsaci </em>

377 views

In Vitro Mineralization Assay: A Colorimetric Method to Quantify Osteoblast Calcium Deposits on Bone Graft Substitute by Alizarin Red S Staining and Extraction

In Vitro Mineralization Assay: A Colorimetric Method to Quantify Osteoblast Calcium Deposits on Bone Graft Substitute by Alizarin Red S Staining and Extraction

1.4K views

Larval Contact Assay: A Procedure to Evaluate Mosquito Larval Mortality following Direct Contact with Test Larvicidal Compound

Larval Contact Assay: A Procedure to Evaluate Mosquito Larval Mortality following Direct Contact with Test Larvicidal Compound

631 views

Topical Testing Assay: A Technique to Evaluate the Insecticidal Potential of Dopamine Receptor Antagonists on Adult Female Mosquitoes by Topical Application

Topical Testing Assay: A Technique to Evaluate the Insecticidal Potential of Dopamine Receptor Antagonists on Adult Female Mosquitoes by Topical Application

491 views

Mosquito Feeding Assay: A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes

Mosquito Feeding Assay: A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes

625 views

Protein Aggregate Formation Assay: A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor

Protein Aggregate Formation Assay: A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor

601 views

Endotoxin Activity Assay: A Chemiluminescence-Based Bioassay for Investigating Endotoxin Activity in Blood Sample

Endotoxin Activity Assay: A Chemiluminescence-Based Bioassay for Investigating Endotoxin Activity in Blood Sample

802 views

Turbidimetric Limulus Amoebocyte Lysate Assay: A Technique to Detect and Quantify Endotoxin Contamination in Nano-Formulated Drugs

Turbidimetric Limulus Amoebocyte Lysate Assay: A Technique to Detect and Quantify Endotoxin Contamination in Nano-Formulated Drugs

588 views

Gel-Clot Limulus Amoebocyte Lysate Assay: A Method for Bacterial Endotoxin Detection in Nanoparticle Formulations

Gel-Clot Limulus Amoebocyte Lysate Assay: A Method for Bacterial Endotoxin Detection in Nanoparticle Formulations

924 views

Chromogenic Polymer Hydrogel-Based Enzyme Screening Assay: A High Throughput Method to Screen the Carbohydrate-Active Enzymes Using Synthetic Substrates

Chromogenic Polymer Hydrogel-Based Enzyme Screening Assay: A High Throughput Method to Screen the Carbohydrate-Active Enzymes Using Synthetic Substrates

431 views

Insoluble Chromogenic Biomass-Based Enzyme Screening: An Assay to Evaluate the Degradation Activity of Plant Biomass-Degrading Enzymes

Insoluble Chromogenic Biomass-Based Enzyme Screening: An Assay to Evaluate the Degradation Activity of Plant Biomass-Degrading Enzymes

397 views

Differential Nuclear Staining Assay: An Assay to Determine Mitocan Cytotoxicity by Propidium Iodide and Hoechst Double Staining

Differential Nuclear Staining Assay: An Assay to Determine Mitocan Cytotoxicity by Propidium Iodide and Hoechst Double Staining

707 views

The Gliding Actin Filament Assay: An In Vitro Motility Assay to Study Translocation of Actin Filaments on Immobilized Myosins

The Gliding Actin Filament Assay: An In Vitro Motility Assay to Study Translocation of Actin Filaments on Immobilized Myosins

1.3K views

Inverted Motility Assay: An In Vitro Technique to Visualize Myosin Movement on Immobilized Actin Filaments

Inverted Motility Assay: An In Vitro Technique to Visualize Myosin Movement on Immobilized Actin Filaments

588 views

Differential Radial Capillary Action of Ligand Assay: A High-Throughput Technique to Identify Bacterial Nucleotide Second Messenger-Binding Intracellular Proteins

Differential Radial Capillary Action of Ligand Assay: A High-Throughput Technique to Identify Bacterial Nucleotide Second Messenger-Binding Intracellular Proteins

415 views

Misfolding-Prone Protein Degradation Assay: A Technique to Monitor Misfolded Protein Degradation Using Cycloheximide Treatment and Detergent Fractionation

Misfolding-Prone Protein Degradation Assay: A Technique to Monitor Misfolded Protein Degradation Using Cycloheximide Treatment and Detergent Fractionation

566 views

Fluorescence Microplate-Based Cycloheximide Chase Assay: A Technique to Monitor the Degradation Kinetics of Fluorescent Nuclear Misfolded Proteins

Fluorescence Microplate-Based Cycloheximide Chase Assay: A Technique to Monitor the Degradation Kinetics of Fluorescent Nuclear Misfolded Proteins

874 views

The Luciferase Assay: A High-Throughput Assay to Measure Luminescent Signals Using Engineered Nanoluciferase System-Based Bioreporters

The Luciferase Assay: A High-Throughput Assay to Measure Luminescent Signals Using Engineered Nanoluciferase System-Based Bioreporters

878 views

Fluorescent Reporter-Based Paralysis Assay: A Technique to Assess Age-Associated Progressive Formation of Polyglutamine Fluorescent Reporter and Associated Paralysis in <em>Caenorhabditis elegans</em>

Fluorescent Reporter-Based Paralysis Assay: A Technique to Assess Age-Associated Progressive Formation of Polyglutamine Fluorescent Reporter and Associated Paralysis in <em>Caenorhabditis elegans</em>

466 views

Amplified Luminescent Proximity Homogeneous Assay: A Bead-Based Proximity Assay to Screen Small Molecules Inhibiting Protein-Protein Interactions

Amplified Luminescent Proximity Homogeneous Assay: A Bead-Based Proximity Assay to Screen Small Molecules Inhibiting Protein-Protein Interactions

448 views

Gut Acidification Monitoring Assay: A Technique to Study the Acidification of the Intestinal Lumen in <em>Drosophila</em> using Bromophenol Blue Dye

Gut Acidification Monitoring Assay: A Technique to Study the Acidification of the Intestinal Lumen in <em>Drosophila</em> using Bromophenol Blue Dye

606 views

Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

1.1K views

Soluble Protein Assay to Quantify Soluble Protein Content in Plant Tissues

Soluble Protein Assay to Quantify Soluble Protein Content in Plant Tissues

801 views

Colorimetric Assay for the Quantification of Non-Structural Carbohydrates in Plant Tissues

Colorimetric Assay for the Quantification of Non-Structural Carbohydrates in Plant Tissues

2.7K views

Luciferase-Based Growth Assay to Quantify Intracellular Parasite Reproduction

Luciferase-Based Growth Assay to Quantify Intracellular Parasite Reproduction

470 views

Luciferase Assay to Study Efficacy of Antiparasitic Chemicals Against Parasites

Luciferase Assay to Study Efficacy of Antiparasitic Chemicals Against Parasites

436 views

Msp1 Extraction Assay to Study the Removal of Mislocalized TA Proteins

Msp1 Extraction Assay to Study the Removal of Mislocalized TA Proteins

315 views

β-Galactosidase Assay to Evaluate Drug-Induced Cytotoxicity in Parasites

β-Galactosidase Assay to Evaluate Drug-Induced Cytotoxicity in Parasites

490 views

A Plate-Based Assay to Measure Monoamine Release from Brain Slices on Test Drug Exposure

A Plate-Based Assay to Measure Monoamine Release from Brain Slices on Test Drug Exposure

354 views

PolyQ Aggregation Assay to Identify Neuroprotective Effect of a Compound in Worms

PolyQ Aggregation Assay to Identify Neuroprotective Effect of a Compound in Worms

557 views

An Assay to Study Effect of Test Compound against PolyQ-Mediated Neurotoxicity in Worms

An Assay to Study Effect of Test Compound against PolyQ-Mediated Neurotoxicity in Worms

345 views

Examine Chemoavoidance Behavior in Transgenic Worms Using Osmotic Avoidance Assay

Examine Chemoavoidance Behavior in Transgenic Worms Using Osmotic Avoidance Assay

497 views

Colorimetric Assay for Quantification of Lactate in Test Samples

Colorimetric Assay for Quantification of Lactate in Test Samples

755 views

Pyruvate Assay for Estimation of Pyruvate Levels in Cellular Fractions of Worms

Pyruvate Assay for Estimation of Pyruvate Levels in Cellular Fractions of Worms

522 views

Spectrophotometric Assay to Determine Glycogen Synthase Activity

Spectrophotometric Assay to Determine Glycogen Synthase Activity

635 views

Glycogen Branching Assay to Measure the Degree of Glycogen Branching

Glycogen Branching Assay to Measure the Degree of Glycogen Branching

509 views

Thiobarbituric Acid-Reactive Substances Assay to Assess Oxidative Stress in Human Serum

Thiobarbituric Acid-Reactive Substances Assay to Assess Oxidative Stress in Human Serum

905 views

Split Luciferase Complementation Assay to Identify Specific Protein-Protein Interactions

Split Luciferase Complementation Assay to Identify Specific Protein-Protein Interactions

735 views

Soil Core Sample Collection for Water Retention Property Analysis

Soil Core Sample Collection for Water Retention Property Analysis

743 views

Controlled Infusion of Radiopharmaceutical Agent Using a Peristaltic Pump

Controlled Infusion of Radiopharmaceutical Agent Using a Peristaltic Pump

68 views

Extracellular Vesicle Uptake Assay: A Method to Visualize and Quantify Cellular Uptake of Extracellular Vesicles Using 3D Fluorescence Imaging Technique

Extracellular Vesicle Uptake Assay: A Method to Visualize and Quantify Cellular Uptake of Extracellular Vesicles Using 3D Fluorescence Imaging Technique

1K views

Three-Chambered Array-Based Impedance Assay: A Real-Time Analysis Technique to Assess Invasive Potential of Cancer Cells by Measuring Electrical Impedance

Three-Chambered Array-Based Impedance Assay: A Real-Time Analysis Technique to Assess Invasive Potential of Cancer Cells by Measuring Electrical Impedance

386 views

Lysostaphin-Based Enzyme Protection Assay: An In Vitro Method to Quantify Intracellular <em> Staphylococcus aureus </em> Load by Enzyme-Mediated Selective Killing of Extracellular Bacteria

Lysostaphin-Based Enzyme Protection Assay: An In Vitro Method to Quantify Intracellular <em> Staphylococcus aureus </em> Load by Enzyme-Mediated Selective Killing of Extracellular Bacteria

555 views

Resorption Pit Assay: An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates

Resorption Pit Assay: An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates

718 views

Protein Phase Separation Assay: An Optogenetic Method for Mutant RNA-Binding Protein Phase Separation in Spinal Motor Neurons of Zebrafish Larvae

Protein Phase Separation Assay: An Optogenetic Method for Mutant RNA-Binding Protein Phase Separation in Spinal Motor Neurons of Zebrafish Larvae

607 views

Bead Sprouting Assay for Early-Stage Angiogenesis Assessment: An In Vitro Bead-Based Model to Study Endothelial Sprouting in the Presence of Pericytes

Bead Sprouting Assay for Early-Stage Angiogenesis Assessment: An In Vitro Bead-Based Model to Study Endothelial Sprouting in the Presence of Pericytes

737 views

Hemolysis Assay: An In Vitro Method to Measure Calcium Ionophore-Induced Lysis in Human Erythrocytes

Hemolysis Assay: An In Vitro Method to Measure Calcium Ionophore-Induced Lysis in Human Erythrocytes

1.3K views

Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling

Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling

879 views

Caco-2 Cell Bioassay: An In Vitro Method for Measuring Iron Bioavailability in Complex Food Sources

Caco-2 Cell Bioassay: An In Vitro Method for Measuring Iron Bioavailability in Complex Food Sources

802 views

In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement

In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement

663 views

Measurement of Extracellular Fluorescent Glucose Analog Depletion: A Surrogate Measurement Technique to Assess Glucose Uptake in Mouse Organs Ex Vivo

Measurement of Extracellular Fluorescent Glucose Analog Depletion: A Surrogate Measurement Technique to Assess Glucose Uptake in Mouse Organs Ex Vivo

537 views

Measuring SMC Contraction In Vitro: A Non-Invasive Method to Evaluate Smooth Muscle Cells Contraction using Electric Cell-Substrate Impedance Sensing

Measuring SMC Contraction In Vitro: A Non-Invasive Method to Evaluate Smooth Muscle Cells Contraction using Electric Cell-Substrate Impedance Sensing

680 views

Humanized Mediator Enzyme Release Assay: An Immunochemical Method to Screen Allergic Potential of Test Antigen by Monitoring Basophil Degranulation Response In Vitro

Humanized Mediator Enzyme Release Assay: An Immunochemical Method to Screen Allergic Potential of Test Antigen by Monitoring Basophil Degranulation Response In Vitro

393 views

ATP-Based Luciferase Viability Assay: A Homogenous Method to Evaluate the Growth-Inhibitory Potential of Test Agents on Tumor Organoids

ATP-Based Luciferase Viability Assay: A Homogenous Method to Evaluate the Growth-Inhibitory Potential of Test Agents on Tumor Organoids

587 views

Fluorescent Probe Imaging Assay: A Technique to Visualize Oxidative Stress in the Reactive Oxygen Species Inducer-Treated Cultured Intestinal Organoid Cells

Fluorescent Probe Imaging Assay: A Technique to Visualize Oxidative Stress in the Reactive Oxygen Species Inducer-Treated Cultured Intestinal Organoid Cells

437 views

Flow Cytometry Assay to Quantify Oxidative Stress: An Assay to Quantify Reactive Oxygen Species in Intestinal Organoids Using Fluorogenic Probes

Flow Cytometry Assay to Quantify Oxidative Stress: An Assay to Quantify Reactive Oxygen Species in Intestinal Organoids Using Fluorogenic Probes

685 views

In Vitro Persister Assay: A Method to Evaluate the Effect of Osmolytes on Bacterial Persistence

In Vitro Persister Assay: A Method to Evaluate the Effect of Osmolytes on Bacterial Persistence

392 views

Bacterial Co-Incubation Assay: A Fluorescence Microscopy-Based Technique to Visualize Intraspecific Bacterial Competition at the Single-Cell Level

Bacterial Co-Incubation Assay: A Fluorescence Microscopy-Based Technique to Visualize Intraspecific Bacterial Competition at the Single-Cell Level

810 views

Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells

Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells

2K views

Terminal Transferase-Mediated dUTP Nick End-Labeling—TUNEL Assay: An In Situ Method to Detect DNA Fragmentation in Apoptotic Cells

Terminal Transferase-Mediated dUTP Nick End-Labeling—TUNEL Assay: An In Situ Method to Detect DNA Fragmentation in Apoptotic Cells

783 views

Caspase-3/7 Assay: A Luminescence-Based Assay to Quantify Apoptosis by Measuring Caspase-3/7 Activities in Frog Skin Explants

Caspase-3/7 Assay: A Luminescence-Based Assay to Quantify Apoptosis by Measuring Caspase-3/7 Activities in Frog Skin Explants

872 views

The Phagocytosis Assay: An Immunostaining Technique to Visualize Apoptotic Cell Engulfment in the Embryonic Cells of <em>Drosophila melanogaster</em>

The Phagocytosis Assay: An Immunostaining Technique to Visualize Apoptotic Cell Engulfment in the Embryonic Cells of <em>Drosophila melanogaster</em>

457 views

Fixed Cell Efferocytosis Assay: A Method to Study Efferocytic Uptake of Apoptotic Cells by Macrophages Using Fluorescence Microscopy

Fixed Cell Efferocytosis Assay: A Method to Study Efferocytic Uptake of Apoptotic Cells by Macrophages Using Fluorescence Microscopy

1.1K views

The In Vitro Single Oocyte Reporter Assay: A Technique to Study Target mRNA Translation Regulation During In Vitro Oocyte Maturation

The In Vitro Single Oocyte Reporter Assay: A Technique to Study Target mRNA Translation Regulation During In Vitro Oocyte Maturation

521 views

alamarBlue Assay: A Sensitive Fluorometric Method to Screen the Cytotoxic Effects of Artificial Tear Formulations on Metabolic Activity of Human Corneal Epithelial Cells

alamarBlue Assay: A Sensitive Fluorometric Method to Screen the Cytotoxic Effects of Artificial Tear Formulations on Metabolic Activity of Human Corneal Epithelial Cells

927 views

Calcium Influx Assay to Measure Mitochondrial Calcium Uptake in Cultured Cells

Calcium Influx Assay to Measure Mitochondrial Calcium Uptake in Cultured Cells

882 views

Fluorescence Leakage Assay to Study Cell-Penetrating Peptide Interaction with Membrane

Fluorescence Leakage Assay to Study Cell-Penetrating Peptide Interaction with Membrane

539 views

Calcification Assay to Measure Calcium Concentration in Aortic Valve Interstitial Cells

Calcification Assay to Measure Calcium Concentration in Aortic Valve Interstitial Cells

456 views

Electroencephalography-Based Recording Technique to Record the Epileptiform Activity

Electroencephalography-Based Recording Technique to Record the Epileptiform Activity

497 views

Wound Assay to Evaluate the Migratory Capacity of Murine Myoblasts in Co-Culture

Wound Assay to Evaluate the Migratory Capacity of Murine Myoblasts in Co-Culture

493 views

Comet Assay for DNA Damage Detection in Cells

Comet Assay for DNA Damage Detection in Cells

2.1K views

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

833 views

Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time

Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time

501 views

Impedance-Based Cellular Assay to Measure Multiplicity of Infection of Influenza Virus

Impedance-Based Cellular Assay to Measure Multiplicity of Infection of Influenza Virus

353 views

Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival

Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival

402 views

Choroid Sprouting Assay to Study Ocular Microvascular Proliferation Ex Vivo

Choroid Sprouting Assay to Study Ocular Microvascular Proliferation Ex Vivo

544 views

Estrogen Receptor-Reporter Activity Assay to Screen Phytoestrogen Estrogenic Activity

Estrogen Receptor-Reporter Activity Assay to Screen Phytoestrogen Estrogenic Activity

446 views

Time-Resolved Forster Resonance Energy Transfer for Monitoring Protein Phosphorylation in Cells

Time-Resolved Forster Resonance Energy Transfer for Monitoring Protein Phosphorylation in Cells

347 views

Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons

Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons

430 views

Assessing Bone Mineral Density Using Radiofrequency Echographic Multi-Spectrometry

Assessing Bone Mineral Density Using Radiofrequency Echographic Multi-Spectrometry

534 views

A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing

A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing

679 views

Oxygen Flux Measurements to Evaluate Mitochondrial Respiration in Living and Permeabilized Cells

Oxygen Flux Measurements to Evaluate Mitochondrial Respiration in Living and Permeabilized Cells

83 views

Multianalyte Immunobead Assay: A Flow-Based Immunoassay Technique for Concurrent Detection of Multiple Antibody Subtypes in Human Serum Sample

Multianalyte Immunobead Assay: A Flow-Based Immunoassay Technique for Concurrent Detection of Multiple Antibody Subtypes in Human Serum Sample

491 views

The Virus-Like Particles Capture Assay: A Method to Isolate Antigen-Displaying VLPs from a Sample Using Neutralizing Antibody-Conjugated Magnetic Beads

The Virus-Like Particles Capture Assay: A Method to Isolate Antigen-Displaying VLPs from a Sample Using Neutralizing Antibody-Conjugated Magnetic Beads

683 views

Single-Molecule Pull-Down Assay for Protein Phosphorylation Analysis: A High Throughput Technique to Quantify Protein Phosphorylation in Cell Lysate

Single-Molecule Pull-Down Assay for Protein Phosphorylation Analysis: A High Throughput Technique to Quantify Protein Phosphorylation in Cell Lysate

763 views

SUMOylation Assay: An In Vitro Technique to Detect the SUMOylation Status of Substrate Proteins by Immunoblotting

SUMOylation Assay: An In Vitro Technique to Detect the SUMOylation Status of Substrate Proteins by Immunoblotting

471 views

Bioreporter Assay: A Sensitive Technique using a Bioluminescent Reporter System to Detect SARS-CoV-2 Antibodies

Bioreporter Assay: A Sensitive Technique using a Bioluminescent Reporter System to Detect SARS-CoV-2 Antibodies

368 views

Cell-Based AAV Neutralizing Antibody Assay: A Colorimetric Technique to Detect Neutralizing Antibodies Against Specific Adeno-Associated Viruses in Serum

Cell-Based AAV Neutralizing Antibody Assay: A Colorimetric Technique to Detect Neutralizing Antibodies Against Specific Adeno-Associated Viruses in Serum

700 views

Immunofluorescence Assay to Detect PM<sub>2.5</sub>-Mediated DNA Damage in Fish Embryos

Immunofluorescence Assay to Detect PM<sub>2.5</sub>-Mediated DNA Damage in Fish Embryos

365 views

Neuronal Death Assay to Evaluate Cell Death in Ex Vivo Epilepsy Model

Neuronal Death Assay to Evaluate Cell Death in Ex Vivo Epilepsy Model

467 views

Enzyme-Linked Immunosorbent Assay to Verify Chemically-Coupled Antibody-Antigen Conjugates

Enzyme-Linked Immunosorbent Assay to Verify Chemically-Coupled Antibody-Antigen Conjugates

531 views

Immunofluorescence Assay to Evaluate Effect of Target Proteins on Neurite Outgrowth

Immunofluorescence Assay to Evaluate Effect of Target Proteins on Neurite Outgrowth

507 views

Electrochemiluminescence Assay for Quantification of Target Protein Levels in Brain Lysate

Electrochemiluminescence Assay for Quantification of Target Protein Levels in Brain Lysate

553 views

Tissue Immunostaining for Imaging Mass Cytometry

Tissue Immunostaining for Imaging Mass Cytometry

763 views

Preparation of Platelet-Rich Plasma from Human Peripheral Blood

Preparation of Platelet-Rich Plasma from Human Peripheral Blood

1.2K views

PCR Techniques

12 Videos · 55 Minutes

Digital Droplet Polymerase Chain Reaction for Indel Mutation Detection in Target Genes

Digital Droplet Polymerase Chain Reaction for Indel Mutation Detection in Target Genes

1.4K views

Gradient Polymerase Chain Reaction to Determine the Optimum Annealing Temperature

Gradient Polymerase Chain Reaction to Determine the Optimum Annealing Temperature

869 views

Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers

Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers

901 views

Real-Time Quantitative Reverse Transcription PCR for Diagnosing Viral Infections

Real-Time Quantitative Reverse Transcription PCR for Diagnosing Viral Infections

1.5K views

Wild-Type Blocking PCR to Detect Low-Frequency Somatic Mutations

Wild-Type Blocking PCR to Detect Low-Frequency Somatic Mutations

603 views

Quantitative Polymerase Chain Reaction to Enumerate Bacteriophages

Quantitative Polymerase Chain Reaction to Enumerate Bacteriophages

846 views

Microsphere Polymerase Chain Reaction to Amplify Single-Stranded DNA

Microsphere Polymerase Chain Reaction to Amplify Single-Stranded DNA

490 views

Nested-PCR to Detect a Specific Viral Genomic Sequence

Nested-PCR to Detect a Specific Viral Genomic Sequence

773 views

High-Resolution Melting PCR to Determine Sequence Variations in Mutant DNA Sequences

High-Resolution Melting PCR to Determine Sequence Variations in Mutant DNA Sequences

505 views

Multiplex Droplet Polymerase Chain Reaction to Detect DNA Methylation in Leukocytes

Multiplex Droplet Polymerase Chain Reaction to Detect DNA Methylation in Leukocytes

432 views

Chip-in-a-Tube-Based Digital PCR for Quantification of Single Nucleotide Variants

Chip-in-a-Tube-Based Digital PCR for Quantification of Single Nucleotide Variants

551 views

Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip

Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip

619 views

Blot Techniques

8 Videos · 44 Minutes

Dot Blot Assay to Quantify Viral Genome

Dot Blot Assay to Quantify Viral Genome

1.2K views

5-Methylcytosine Dot Blot to Determine the Extent of DNA Methylation

5-Methylcytosine Dot Blot to Determine the Extent of DNA Methylation

1.1K views

Quantitative Dot Blot to Estimate Target Proteins in a Tissue Lysate

Quantitative Dot Blot to Estimate Target Proteins in a Tissue Lysate

1.2K views

Quantitative Western Blotting to Estimate Protein Expression Levels

Quantitative Western Blotting to Estimate Protein Expression Levels

2.2K views

Single-Cell Western Blotting to Determine Cell Heterogeneity

Single-Cell Western Blotting to Determine Cell Heterogeneity

677 views

Chemiluminescent Western Blot Assay for Protein Processing

Chemiluminescent Western Blot Assay for Protein Processing

1.2K views

Southern Blotting to Detect Homologous Recombination in the Mouse Embryonic Stem Cell Genome

Southern Blotting to Detect Homologous Recombination in the Mouse Embryonic Stem Cell Genome

971 views

Northern Blot to Detect and Quantify Specific MicroRNA in Total RNA Extract of Plant Tissue

Northern Blot to Detect and Quantify Specific MicroRNA in Total RNA Extract of Plant Tissue

1.2K views

Biomolecular Interaction Detection Techniques

44 Videos · 221 Minutes

Graphene Biosensor-Based Field-Effect Biosensing to Detect Protein-Protein Interactions

Graphene Biosensor-Based Field-Effect Biosensing to Detect Protein-Protein Interactions

646 views

Mass Photometry to Study Antigen-Antibody Interactions on a Single-Molecule Level

Mass Photometry to Study Antigen-Antibody Interactions on a Single-Molecule Level

560 views

FLIM-FRET Imaging for Characterization of Protein-Protein Interactions in Live Bacteria

FLIM-FRET Imaging for Characterization of Protein-Protein Interactions in Live Bacteria

615 views

Cell Aggregation Assay to Detect Trans Interactions between Cell Adhesion Molecules

Cell Aggregation Assay to Detect Trans Interactions between Cell Adhesion Molecules

773 views

Proximity Ligation Assay to Study In Situ Protein-Protein Interactions

Proximity Ligation Assay to Study In Situ Protein-Protein Interactions

992 views

On-Membrane Protein Digestion to Prepare Co-Immunoprecipitated Proteins for Interaction Studies

On-Membrane Protein Digestion to Prepare Co-Immunoprecipitated Proteins for Interaction Studies

480 views

Biolayer Interferometry Technology to Detect Interactions between Ligand and Analyte

Biolayer Interferometry Technology to Detect Interactions between Ligand and Analyte

667 views

Fluorescence Fluctuation Spectroscopy to Study Protein Interaction at Cell Contacts

Fluorescence Fluctuation Spectroscopy to Study Protein Interaction at Cell Contacts

366 views

Immunoprecipitation Assay to Study Enzyme Receptor Interactions in Transfected Cells

Immunoprecipitation Assay to Study Enzyme Receptor Interactions in Transfected Cells

421 views

ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins

ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins

1.3K views

β-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions

β-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions

591 views

Biotinylated Cell-Penetrating Peptide Probe to Detect Protein-Protein Interactions

Biotinylated Cell-Penetrating Peptide Probe to Detect Protein-Protein Interactions

425 views

Yeast Two-Hybrid Assay to Determine Protein Self-Association in Yeast Cells

Yeast Two-Hybrid Assay to Determine Protein Self-Association in Yeast Cells

3.9K views

Tandem Affinity Purification Assay to Study Protein-Protein Interactions

Tandem Affinity Purification Assay to Study Protein-Protein Interactions

602 views

Surface Plasmon Resonance to Study Biomolecular Interactions Using a Sensor Chip

Surface Plasmon Resonance to Study Biomolecular Interactions Using a Sensor Chip

806 views

Nuclear Magnetic Resonance to Study Atomic Level Protein-Protein Interactions

Nuclear Magnetic Resonance to Study Atomic Level Protein-Protein Interactions

504 views

Using Phage Display to Select Proteins with High Affinity to a Target Protein

Using Phage Display to Select Proteins with High Affinity to a Target Protein

1.5K views

Fluorescence Fluctuation Spectroscopy to Study Protein Homo-Oligomerization

Fluorescence Fluctuation Spectroscopy to Study Protein Homo-Oligomerization

381 views

Bimolecular Fluorescence Complementation-Coupled Photoactivated Localization Microscopy

Bimolecular Fluorescence Complementation-Coupled Photoactivated Localization Microscopy

342 views

Fluorescence Anisotropy-Based Detection of Protein-Protein Interactions

Fluorescence Anisotropy-Based Detection of Protein-Protein Interactions

531 views

Quantitative In-Cell Hydrogen NMR Spectroscopy to Monitor Protein-Ligand Interactions

Quantitative In-Cell Hydrogen NMR Spectroscopy to Monitor Protein-Ligand Interactions

355 views

Quartz-Crystal Microbalance Biosensor-Based Biopanning to Study Protein-Drug Interactions

Quartz-Crystal Microbalance Biosensor-Based Biopanning to Study Protein-Drug Interactions

525 views

Size-Exclusion Chromatography with Multi-Angle Light Scattering for Protein Oligomers

Size-Exclusion Chromatography with Multi-Angle Light Scattering for Protein Oligomers

618 views

Bimolecular Complementation Affinity Purification to Isolate Two Interacting Proteins

Bimolecular Complementation Affinity Purification to Isolate Two Interacting Proteins

319 views

Competitive Binding Assay to Identify Compounds Disrupting Receptor-Ligand Interactions

Competitive Binding Assay to Identify Compounds Disrupting Receptor-Ligand Interactions

662 views

ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells

ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells

531 views

Biomembrane Force Probe to Quantitate Receptor-Ligand Interactions

Biomembrane Force Probe to Quantitate Receptor-Ligand Interactions

358 views

Competition Binding Assay to Study Competing GTPase-Binding Protein Partners

Competition Binding Assay to Study Competing GTPase-Binding Protein Partners

397 views

Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen

Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen

397 views

Electrophoretic Mobility Shift Assay for Detection of Transcription Factor-DNA Complexes

Electrophoretic Mobility Shift Assay for Detection of Transcription Factor-DNA Complexes

748 views

Chromatin Immunoprecipitation to Identify Target Protein Binding Sites on Genomic DNA

Chromatin Immunoprecipitation to Identify Target Protein Binding Sites on Genomic DNA

729 views

Fluorescence Anisotropy to Determine Transcription Factor-DNA Binding Affinity

Fluorescence Anisotropy to Determine Transcription Factor-DNA Binding Affinity

484 views

Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene

Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene

546 views

Modified Yeast-One Hybrid Assay to Detect Heteromeric Protein Complex-DNA Interactions

Modified Yeast-One Hybrid Assay to Detect Heteromeric Protein Complex-DNA Interactions

451 views

Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes

Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes

332 views

Immunofluorescence-Based Visualization of DNA Repair Protein Interactions

Immunofluorescence-Based Visualization of DNA Repair Protein Interactions

443 views

RNA-Protein Pull-Down Assay to Isolate RNA-Binding Proteins via Affinity Extraction

RNA-Protein Pull-Down Assay to Isolate RNA-Binding Proteins via Affinity Extraction

919 views

SELEX-Based In Vitro Binding Assay to Identify RNA-Protein Interactions

SELEX-Based In Vitro Binding Assay to Identify RNA-Protein Interactions

613 views

Single-Molecule Imaging to Visualize Fusion Protein Condensates Assembly on DNA Curtains

Single-Molecule Imaging to Visualize Fusion Protein Condensates Assembly on DNA Curtains

503 views

Differential Scanning Calorimetry to Study DNA Aptamer-Thermolabile Ligand Interaction

Differential Scanning Calorimetry to Study DNA Aptamer-Thermolabile Ligand Interaction

356 views

Microscale Thermophoresis to Study Protein-Lipid Interactions in Solution

Microscale Thermophoresis to Study Protein-Lipid Interactions in Solution

567 views

Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions

Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions

417 views

Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction

Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction

279 views

pH Modulation Assay on Supported Lipid Bilayers to Detect Protein-Phosphoinositide Interactions

pH Modulation Assay on Supported Lipid Bilayers to Detect Protein-Phosphoinositide Interactions

336 views

Spectroscopy Techniques

6 Videos · 24 Minutes

Nuclear Magnetic Resonance Spectroscopy to Identify Multiple Phosphorylations in Proteins

Nuclear Magnetic Resonance Spectroscopy to Identify Multiple Phosphorylations in Proteins

560 views

Diffuse Correlation Spectroscopy to Assess Cerebral Blood Flow in a Mouse Model

Diffuse Correlation Spectroscopy to Assess Cerebral Blood Flow in a Mouse Model

490 views

The Circular Dichroism Spectroscopy Technique to Study DNA-Protein Interactions

The Circular Dichroism Spectroscopy Technique to Study DNA-Protein Interactions

1.1K views

A Raman Spectroscopy-Based Direct Immunoassay to Detect a Specific Antigen

A Raman Spectroscopy-Based Direct Immunoassay to Detect a Specific Antigen

533 views

UV-Visible Spectroscopy for Monitoring Fibrin Clot Formation

UV-Visible Spectroscopy for Monitoring Fibrin Clot Formation

982 views

A Fourier Transform Infrared Spectroscopy Technique to Study Peptide Self-Assembly

A Fourier Transform Infrared Spectroscopy Technique to Study Peptide Self-Assembly

1.2K views

Microscopy Techniques

10 Videos · 60 Minutes

TIRF Microscopy to Visualize Actin and Microtubule Coupling Dynamics

TIRF Microscopy to Visualize Actin and Microtubule Coupling Dynamics

1.4K views

Lattice Light Sheet Microscopy to Visualize Receptor-Ligand Interactions in Live Cells

Lattice Light Sheet Microscopy to Visualize Receptor-Ligand Interactions in Live Cells

545 views

Darkfield Microscopy to Visualize Diffusional Dynamics of Nanorods on Cell Membrane

Darkfield Microscopy to Visualize Diffusional Dynamics of Nanorods on Cell Membrane

543 views

Interference Reflection Microscopy for Label-Free Visualization of Microtubule Dynamics

Interference Reflection Microscopy for Label-Free Visualization of Microtubule Dynamics

511 views

Static Atomic Force Microscopy to Visualize and Characterize Assembled Nucleosomes

Static Atomic Force Microscopy to Visualize and Characterize Assembled Nucleosomes

507 views

High-Speed Time-Lapse Atomic Force Microscopy to Capture Nucleosome Dynamics

High-Speed Time-Lapse Atomic Force Microscopy to Capture Nucleosome Dynamics

472 views

Transmission Electron Microscopy to Quantify Glycogen Distribution in Human Skeletal Muscles

Transmission Electron Microscopy to Quantify Glycogen Distribution in Human Skeletal Muscles

744 views

Quantification of Membrane Ruffle Formation Using Scanning Electron Microscopy

Quantification of Membrane Ruffle Formation Using Scanning Electron Microscopy

592 views

Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation

Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation

726 views

Immunofluorescence Microscopy for the Analysis of DNA Double-Strand Breaks

Immunofluorescence Microscopy for the Analysis of DNA Double-Strand Breaks

804 views

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