Colony Color Screening
Colony Color Screening - Related Videos
10:27
Training Synesthetic Letter-color Associations by Reading in Color
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Cited by 5 •
2014
Reading in color is a new method for training letter-color associations that are typically found only in grapheme-color synesthetes. It involves an implicit form of training that has potential for long-term associative training methods because the training is a byproduct of reading and any text can be colored.
09:20
Color Afterimages
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Human color vision is impressive. People with normal color vision can tell apart millions of individual hues. Most amazingly, this ability is achieved with fairly simple hardware. Part of the power of human color vision comes from a clever bit of engineering in the human brain. There, color perception relies on what is known as an 'opponent system.' This means that the presence of one kind of stimulus is treated as evidence for...
03:02
Colors and Magnetism
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2020
Color in Coordination Complexes When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human eye.
07:47
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
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Cited by 66 •
2014
ScanLag is a high-throughput method for measuring the delay in growth, namely lag time, as well as the growth rate of colonies for thousands of cells in parallel. Screening using ScanLag enables the discrimination between long lag-time and slow growth at the level of a single variant.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
FAQs
A reporter system links visible pigmentation to the activity of a gene product. When that product is active, it converts an indicator substrate in the agar into a colored compound. If the relevant gene is disrupted or its activity changes, the conversion differs, producing a contrasting colony appearance that helps distinguish candidate cells.
Selective agar provides the growth environment in which colonies carrying the relevant genetic properties can be examined alongside a color response. The selection and the reporter readout serve different purposes: growth helps identify cells meeting the selection condition, while pigmentation or color change supplies an additional visual distinction for sorting colonies during analysis.
Color provides a rapid first-pass indication of a colony’s genetic state, but it does not establish the exact DNA sequence or fully confirm the intended construct. Differences in reporter activity may reflect gene disruption or altered activity. Researchers therefore use the visual result to prioritize colonies for confirmatory testing, such as PCR or sequencing.
Colony Color Screening gives an immediate visual comparison across many colonies, making it efficient for initial selection. PCR and sequencing provide confirmatory information that the color readout cannot supply. In recombinant DNA workflows, the visual method narrows the candidates, while those molecular tests determine whether the selected colony has the expected genetic arrangement or sequence.
Researchers grow bacterial cells on selective agar containing the appropriate reporter system or indicator substrate, then examine the resulting colonies for pigmentation or contrasting color changes. Colonies with the desired appearance become candidates for further analysis. The workflow uses visual classification first and reserves PCR or sequencing for confirmation of selected colonies.
The method is useful when a study requires rapid examination of many bacterial colonies during plasmid cloning, recombinant DNA analysis, or microbial genetics experiments. Its visual readout supports efficient first-pass sorting before more detailed tests. By reducing the number of colonies requiring confirmation, it helps organize downstream analysis without replacing molecular verification.