Endophytic Bacteria
Endophytic Bacteria - Related Videos
01:22
Root-Mediated Endophytic Colonization of Beneficial Bacteria in Rice
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2025
Begin with rice seedlings pre-germinated under dark, aseptic conditions to ensure uniform sprouting.Transfer these seedlings to a flask containing a nutrient-rich, semi-solid medium pre-inoculated with a test bacterium.As the plant grows, the roots release chemotactic compounds into the surrounding medium.These compounds stimulate bacterial proliferation, leading to adhesion, multiplication, and colony formation on the root surface.As the roots develop, the bacteria enter the plant through...
15:14
Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
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Cited by 197 •
2013
This protocol demonstrates two inoculation methods to introduce the fungal entomopathogen Beauveria bassiana as an endophyte in the common bean (Phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control.
01:32
Enhancing Salt Stress Tolerance in Tomato Plants Through Endophytic Bacterial Colonization
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2025
Endophytic bacteria colonize plant tissues and promote plant growth.To study their impact on tomato plant growth under salt stress, start with sterile seedlings showing developing leaves and roots.Immerse the roots of the experimental seedling in a suspension of endophytic bacteria; leave the control uninoculated.Transfer both groups into sterilized soil to prevent external microbial interference and incubate in a growth chamber.From the soil, the bacteria enter through transient root openings,...
06:53
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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Cited by 2 •
2023
The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.
13:20
Detection of Bacteria Using Fluorogenic DNAzymes
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Cited by 18 •
2012
We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental...
FAQs
Chemical signals allow the bacteria and plant to influence one another after internal colonization. These signals help coordinate exchanges involving nutrients and growth-regulating compounds, linking microbial activity with plant development and tissue responses. In biology research, examining this communication helps explain why some bacterial associations support healthy growth rather than producing apparent disease.
These sites provide entry points through which bacteria can reach internal plant spaces. Once inside, their effects depend on interactions with plant cells and available nutrients, not simply on external contact with the plant surface. Comparing these entry routes helps researchers study how internal colonization begins and how bacterial partnerships become established in different plant tissues.
They may support plant protection through two complementary mechanisms. Antimicrobial activity can directly inhibit pathogens, while competition can limit pathogen access to space or resources within plant tissues. The same associations may enhance tolerance to drought or other stresses, making these bacteria relevant for studying how microbial partners contribute to plant resilience under unfavorable conditions.
Nutrient exchange can improve the plant's access to resources, while bacterial growth-regulating compounds can influence developmental processes. Together, these effects connect microbial colonization with outcomes such as improved plant development and nutrient acquisition. Researchers examine this relationship to determine how internal bacterial partners affect plant performance without relying only on external fertilizers or treatments.
Research focuses on linking bacterial associations with measurable plant outcomes, including development, nutrient acquisition, stress tolerance, and pathogen suppression. These findings can guide the selection or development of microbial treatments intended to improve plant health. The agricultural goal is to translate biological interactions observed in plants into practical approaches that reduce reliance on synthetic inputs.
Such treatments are relevant when the objective is to support plant growth, improve nutrient use, or increase tolerance to drought and other stresses. They may also be considered for biological crop protection because antimicrobial activity and competition can suppress pathogens. Their broader value lies in combining plant-health benefits with more sustainable agricultural management strategies.
Their ability to influence nutrient acquisition and plant development supports investigation of biological fertilizers, while antimicrobial activity and competition support biological crop-protection strategies. These applications use naturally occurring plant-microbe interactions rather than depending exclusively on synthetic inputs. Biology research therefore connects internal bacterial partnerships with approaches aimed at maintaining plant health and improving agricultural sustainability.