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JoVE Journal
Immunology and Infection

This content is Open Access.

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Article DOI: 10.3791/3961-v 13:20 min May 28th, 2012
May 28th, 2012



We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental procedures where a specific DNAzyme probe denoted "RFD-EC1" is employed for the detection of the model bacterium, Escherichia coli (E. coli).


Bacteria Detection Fluorogenic DNAzymes Outbreaks Food-borne Pathogens Hospital-acquired Pathogens Analytical Methods Pathogen Detection High Specificity High Sensitivity Short Time-to-results Operational Simplicity Cost Effectiveness Classical Microbiological Methods PCR-based Techniques Antibody-based Techniques Scientific Research
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