Capillary Formation Assay
Capillary Formation Assay - Related Videos
07:42
The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
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Cited by 92 •
2017
The CApillary FEeder (CAFE) assay is a simple, budget-friendly, highly reliable method for investigating mechanisms underlying food intake. Used with the highly versatile genetic model organism Drosophila melanogaster, it provides a powerful means of gaining new insights into regulatory mechanisms of food intake.
08:20
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
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Cited by 9 •
2016
Here, we demonstrate a simple production method for size-controllable, monodisperse, water-in-oil (W/O) microdroplets using a capillary-based centrifugal microfluidic device. This method requires only a small sample volume and enables high-yield production. We expect this method will be useful for rapid biochemical and cellular analyses.
04:18
The CApillary FEeder (CAFE) Assay: A Method to Track Food Consumption and Preference in Drosophila
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2023
This video describes the CApillary FEeder, or CAFE, assay, a behavioral method that measures food intake and preference of unrestrained fruit flies. The featured protocol shows how to perform the assay with minimal evaporation from the liquid-filled capillaries used to deliver the food and track its intake.
03:57
Microtiter Dish Biofilm Formation Assay
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Cited by 2243 •
2011
The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.
08:25
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Cited by 6 •
2017
The genotyping technique described here, which couples fluorescent polymerase chain reaction (PCR) to capillary gel electrophoresis, allows for high-throughput genotyping of nuclease-mediated knockout clones. It circumvents limitations faced by other genotyping techniques and is more cost effective than sequencing methods.
FAQs
The basement membrane matrix provides the surface on which endothelial-cell behavior can be observed, while assay timing determines when organization is evaluated. Cell type and matrix conditions also influence the resulting structures. Because these variables can change migration, alignment, and network appearance, they must be considered when comparing experimental conditions or interpreting differences between samples.
Signals from tumors can be examined through tumor-derived growth factors or conditioned media, allowing investigators to test whether material associated with a tumor changes endothelial organization. Test compounds can be evaluated as experimental variables in the same model. Comparing these conditions helps connect cancer-related signaling with vascularization-related behavior without relying only on tumor-cell observations.
Network length, branch points, and mesh number provide distinct quantitative readouts of the structures formed by endothelial cells. These measurements convert visible differences in organization into values that can be compared across experimental conditions. In cancer research, such comparisons help assess whether tumor-associated factors or candidate compounds alter the extent or pattern of network formation.
The workflow places endothelial cells onto a basement membrane matrix and allows them to organize under the selected assay conditions. During the observation period, cells migrate and align into interconnected structures. Investigators then quantify features such as network length, branch points, or mesh number, making the procedure suitable for comparing untreated, tumor-associated, and compound-exposed conditions.
It is useful when researchers need to evaluate how tumor-derived growth factors or conditioned media influence endothelial organization in a controlled in vitro setting. The assay can also support assessment of candidate antiangiogenic therapies by comparing network outcomes after compound exposure. These applications connect tumor-associated signals with vascularization-related effects and provide measurable experimental endpoints.
These parameters can affect the observed organization independently of the tumor-derived material or test compound being studied. Differences in cell type, matrix conditions, or assay timing may therefore change migration, alignment, and network measurements. Keeping them consistent strengthens comparisons between experimental groups and helps investigators interpret whether an observed outcome reflects the intended biological treatment.